Rapid detection of porcine circovirus type 2 by a red latex microsphere immunochromatographic strip.
Rapid detection of porcine circovirus type 2 by a red latex microsphere immunochromatographic strip.
复制标题
红色乳胶微球免疫层析试纸条快速检测猪圆环病毒2型
DOI：
10.1007/s00253-022-12074-y
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发表时间：
2022-09
影响因子：
5
通讯作者：
Huang, Liping
中科院分区：
文献类型：
--
作者：
Yu, Chong;Wei, Yanwu;Zhang, Hao;Liu, Jianhang;Feng, Li;Liu, Changming;Huang, Liping
为建立猪圆环病毒2型（PCV2）的快速特异性抗原检测方法，制备了针对PCV2流行株的单克隆抗体（mab），并建立了红乳胶微球免疫层析试纸条。共制备了8株抗PCV2b和4株抗PCV2d单克隆抗体，其中7株单克隆抗体通过免疫过氧化物酶单层实验证实能与PCV2a、PCV2b和PCV2d菌株发生反应。微中和试验结果表明，单抗2C8、9H4、10G7、7B9和7C7具有良好的中和活性，而单抗4B3、4C9、6H9和7E2的中和活性均低于50%。选择4B3、7C7、9H4 3个单抗和PCV2单抗建立红胶乳微球免疫层析条带，其中红胶乳微球标记的单抗7C7与单抗9H4组合检测能力最强。免疫层析条对PCV2a/CL、PCV2b/MDJ和PCV2d/LNHC的最低检出限分别为102.5 TCID50/0.1 ml、100.7 TCID50/0.1 ml和101.5 TCID50/0.1 ml。非洲猪瘟病毒、猪瘟病毒、猪呼吸与生殖综合征病毒、猪细小病毒、猪伪狂犬病毒、猪圆环病毒1型、传染性胃肠炎病毒、猪流行性腹泻病毒、猪轮状病毒、猪三角冠状病毒免疫层析条未发现交叉反应性。以PCR为参比标准，免疫层析条的检测灵敏度、特异性和总符合率分别为81.13%、100%和90.00%。此外，免疫层析条的检测能力与病毒滴定的检测能力相关。应用免疫层析条检测183份临床疾病样本，平均阳性检出率为22.95%。综上所述，该方法具有良好的灵敏度和特异性，操作简单、方便、快捷。对PCV2的现场诊断和病毒定量分析具有较高的应用价值。研制了一种用于PCV2检测的红色乳胶微球免疫层析试纸条。•该方法不仅操作简单，而且耗时短。•该方法具有良好的灵敏度和特异性。
To establish a rapid and specific antigen detection method for porcine circovirus type 2 (PCV2), monoclonal antibodies (mAbs) were produced against the PCV2 epidemic strains and a red latex microsphere immunochromatographic strip was established. A total of eight anti-PCV2b and four anti-PCV2d mAbs were produced, and seven mAbs were confirmed to react with PCV2a, PCV2b, and PCV2d strains using an immunoperoxidase monolayer assay. The results of micro-neutralization tests showed that the mAbs 2C8, 9H4, 10G7, 7B9, and 7C7 had good neutralizing activity, whereas the neutralizing activity of the mAbs 4B3, 4C9, 6H9, and 7E2 was lower than 50%. Three mAbs, 4B3, 7C7, and 9H4, and PCV2 pAb were selected for the establishment of a red latex microsphere immunochromatographic strip, and the combination of mAb 7C7 labeled with red latex microspheres and mAb 9H4 exhibited the greatest detection ability. The immunochromatographic strip had minimum detection limits of 102.5 TCID50/0.1 ml, 100.7 TCID50/0.1 ml, and 101.5 TCID50/0.1 ml for PCV2a/CL, PCV2b/MDJ, and PCV2d/LNHC, respectively. Furthermore, no cross-reactivity was found for African swine fever virus, classical swine fever virus, porcine respiratory and reproductive syndrome virus, porcine parvovirus, porcine pseudorabies virus, porcine circovirus type 1, transmissible gastroenteritis virus, porcine epidemic diarrhea virus, porcine rotavirus, or porcine deltacoronavirus using the immunochromatographic strip. Using PCR as a reference standard, the detection sensitivity, specificity, and overall coincidence rate of the immunochromatographic strip were 81.13%, 100%, and 90.00%. Additionally, the detection ability of the immunochromatographic strip was correlated with that of virus titration. The immunochromatographic strip was used to detect 183 clinical disease samples, and the average positive detection rate was 22.95%. In summary, this method has good sensitivity and specificity and is simple, convenient, and quick to operate. It has high application value for on-site diagnosis of PCV2 and virus quantification. • A red latex microsphere immunochromatographic strip for PCV2 detection was developed. • The method was not only simple to operate, but also takes less time. • The method had good sensitivity and specificity.
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影响因子：
2.7
作者：
Liu J;Wei C;Dai A;Lin Z;Fan K;Fan J;Liu J;Luo M;Yang X
通讯作者：
Yang X
影响因子：
4.4
作者：
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影响因子：
3.3
作者：
Blunt, R.;McOrist, S.;Mellits, K.
通讯作者：
Mellits, K.
影响因子：
3.1
作者：
Kim YK;Lim SI;Cho IS;Cheong KM;Lee EJ;Lee SO;Kim JB;Kim JH;Jeong DS;An BH;An DJ
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开发用于检测猪圆环病毒 2 型血清中和抗体的封闭 ELISA
影响因子：
3.1
作者：
Huang, Liping;Lu, Yuehua;Liu, Changming
通讯作者：
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