A novel diagnostic approach to detecting porcine epidemic diarrhea virus: The lateral immunochromatography assay.
A novel diagnostic approach to detecting porcine epidemic diarrhea virus: The lateral immunochromatography assay.
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DOI：
10.1016/j.jviromet.2015.08.024
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发表时间：
2015-12-01
影响因子：
3.1
通讯作者：
An DJ
中科院分区：
文献类型：
--
作者：
Kim YK;Lim SI;Cho IS;Cheong KM;Lee EJ;Lee SO;Kim JB;Kim JH;Jeong DS;An BH;An DJ
建立了一种新的检测PEDV抗原的免疫层析方法。与实时荧光定量PCR相比，IC检测的敏感性为96.0%，特异性为98.5%。PEDV的检测限为1 × 103拷贝。IC测定可在4 °C或室温下储存15个月而不影响其功效。猪流行性腹泻病毒（PEDV）可引起哺乳仔猪急性腹泻和脱水，死亡率高。免疫层析（IC）测定，称为侧流试验，是一种用于检测目标病原体存在的简单装置。在这里，我们开发了一种IC检测PEDV抗原与96.0%（218/227）的灵敏度和98.5%（262/266）的特异性相比，实时逆转录酶（RT）-PCR使用FAM标记的探针的基础上，从核衣壳基因序列。荧光定量RT-PCR和IC法的检测限分别为1 × 102和1 × 103拷贝。本文开发的IC测定法未检测到与其他病毒或细菌病原体的非特异性反应，并且该测定法可在4 °C或室温下储存15个月而不影响其功效。因此，IC测定可导致农场上改进的PED检测和控制，并且是PEDV的当前诊断工具的可行替代方案。
A novel lateral immunochromatography (IC) assay was developed to detect PEDV antigen. The IC assay has 96.0% sensitivity and 98.5% specificity compared with real-time reverse transcriptase PCR. The detection limit for PEDV was 1 × 103 copies. The IC assay could be stored at 4 °C or room temperature for 15 months without affecting its efficacy. Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in sucking piglets and has a high mortality rate. An immunochromatography (IC) assay, known as a lateral flow test, is a simple device intended to detect the presence of target pathogens. Here, we developed an IC assay that detected PEDV antigens with 96.0% (218/227) sensitivity and 98.5% (262/266) specificity when compared with real-time reverse transcriptase (RT)-PCR using FAM-labeled probes based on sequences from nucleocapsid genes. The detection limits of the real-time RT-PCR and IC assays were 1 × 102 and 1 × 103 copies, respectively. The IC assay developed herein did not detect non-specific reactions with other viral or bacterial pathogens, and the assay could be stored at 4 °C or room temperature for 15 months without affecting its efficacy. Thus, the IC assay may result in improved PED detection and control on farms, and is a viable alternative to current diagnostic tools for PEDV.
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影响因子：
2.2
作者：
Klingenberg, KD;Esfandiari, J
通讯作者：
Esfandiari, J
影响因子：
12.6
作者：
Meng, Kai;Sun, Wenjing;Chai, Tongjie
通讯作者：
Chai, Tongjie
影响因子：
11.8
作者：
Puranaveja S;Poolperm P;Lertwatcharasarakul P;Kesdaengsakonwut S;Boonsoongnern A;Urairong K;Kitikoon P;Choojai P;Kedkovid R;Teankum K;Thanawongnuwech R
通讯作者：
Thanawongnuwech R
影响因子：
6.2
作者：
Kim YA;Lee EH;Kim KO;Lee YT;Hammock BD;Lee HS
通讯作者：
Lee HS
DOI：
10.3891/acta.chem.scand.50-0141
发表时间：
1996-02-01
期刊：
ACTA CHEMICA SCANDINAVICA
影响因子：
--
作者：
Laitinen, MPA;Vuento, M
通讯作者：
Vuento, M