Genetic Dissection and Validation of Chromosomal Regions for Transmission Ratio Distortion in Intersubspecific Crosses of Rice.
Genetic Dissection and Validation of Chromosomal Regions for Transmission Ratio Distortion in Intersubspecific Crosses of Rice.
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水稻亚种间杂交染色体区域传递比畸变的遗传剖析和验证
DOI：
10.3389/fpls.2020.563548
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发表时间：
2020
影响因子：
5.6
通讯作者：
Yu S
中科院分区：
文献类型：
--
作者：
Zhang C;Wang D;Wang J;Sun Q;Tian L;Tang X;Yuan Z;He H;Yu S
传输率失真（TRD）是指一个等位基因通过杂合子比另一个等位基因更频繁地传递给后代的普遍现象。TRD被认为是存在生殖障碍的标志。然而，水稻TRD的遗传和分子机制在很大程度上仍然未知。以粳稻品种Nipponbare （NIP）与籼稻品种9311杂交而成的回交自交系（BILs）群体为材料，研究了TRD的遗传基础。在BILs中共鉴定出18个与TRD相关的基因组区域。其中籼稻（9311）和粳稻（NIP）分别有12个和6个区域具有优先传播的等位基因。利用一系列F2群体来证实TRD效应，包括6个基因组区域，这些区域是由亚种间等位基因组合的染色体片段代换系（CSSL）衍生的F2群体所证实的。然而，这些区域都没有被来自亚特异性内等位基因组合的cssl衍生群体所证实。此外，TRD1.3和TRD8.1之间存在显著的上位交互作用，表明TRD对打破亚种间生殖障碍有积极作用。本研究结果为鉴定TRD基因奠定了基础，为广泛亲和品种的选育提供了有效的策略，并将在亚种间杂交育种中得到进一步的应用。
Transmission ratio distortion (TRD) refers to a widespread phenomenon in which one allele is transmitted by heterozygotes more frequently to the progeny than the opposite allele. TRD is considered as a mark suggesting the presence of a reproductive barrier. However, the genetic and molecular mechanisms underlying TRD in rice remain largely unknown. In the present study, a population of backcross inbred lines (BILs) derived from the cross of a japonica cultivar Nipponbare (NIP) and an indica variety 9311 was utilized to study the genetic base of TRD. A total of 18 genomic regions were identified for TRD in the BILs. Among them, 12 and 6 regions showed indica (9311) and japonica (NIP) alleles with preferential transmission, respectively. A series of F2 populations were used to confirm the TRD effects, including six genomic regions that were confirmed by chromosome segment substitution line (CSSL)-derived F2 populations from intersubspecific allelic combinations. However, none of the regions was confirmed by the CSSL-derived populations from intrasubspecific allelic combination. Furthermore, significant epistatic interaction was found between TRD1.3 and TRD8.1 suggesting that TRD could positively contribute to breaking intersubspecific reproductive barriers. Our results have laid the foundation for identifying the TRD genes and provide an effective strategy to breakdown TRD for breeding wide-compatible lines, which will be further utilized in the intersubspecific hybrid breeding programs.
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影响因子：
7
作者：
Huang, Xuehui;Feng, Qi;Han, Bin
通讯作者：
Han, Bin
影响因子：
30.8
作者：
Bauer, H;Willert, JR;Herrmann, BG
通讯作者：
Herrmann, BG
影响因子：
3.3
作者：
Fishman, L;Willis, JH
通讯作者：
Willis, JH
三个代表性的亚种间和亚种内杂交揭示了水稻生殖隔离的遗传结构
影响因子：
7.2
作者：
Li, Guangwei;Li, Xiaoting;Ouyang, Yidan
通讯作者：
Ouyang, Yidan
影响因子：
11.8
作者：
Kusano, A;Staber, C;Ganetzky, B
通讯作者：
Ganetzky, B