Soy biodiesel and petrodiesel emissions differ in size, chemical composition and stimulation of inflammatory responses in cells and animals.
Soy biodiesel and petrodiesel emissions differ in size, chemical composition and stimulation of inflammatory responses in cells and animals.
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DOI：
10.1021/es403146c
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发表时间：
2013
影响因子：
11.4
通讯作者：
Holmen, Britt A.
中科院分区：
文献类型：
--
作者：
Fukagawa, Naomi K.;Li, Muyao;Poynter, Matthew E.;Palmer, Brian C.;Parker, Erin;Kasumba, John;Holmen, Britt A.
由于废气产生方法以及用于评估反应的生物模型存在差异，关于生物柴油废气排放的生物效应存在争议。因为细胞研究不一定能反映整个动物的综合反应，所以利用大众轻型柴油发动机使用石油柴油（B0）和生物柴油混合物（B20：按体积计20%大豆生物柴油/80% B0）产生的原始废气的相同颗粒悬浮液，在两种代表支气管上皮细胞和巨噬细胞的人类细胞系以及雌性小鼠中进行了实验。尾气颗粒排放测量显示，B0产生的颗粒质量是B20燃料的两倍多，具有更大的超细颗粒数量分布模式，且颗粒的非极性有机成分更多。生物学检测（炎症介质、氧化应激生物标志物）表明，两种燃料燃烧产生的颗粒物（PM）在体外和体内模型中诱导了不同的反应。B20处理的细胞培养基以及暴露于B20的小鼠支气管肺泡灌洗液中的炎症介质（白细胞介素 - 6，IL - 6；干扰素 - γ诱导蛋白10，IP - 10；粒细胞刺激因子，G - CSF）浓度比对照或B0 PM高约20 - 30%，这表明在柴油燃料中添加生物柴油会减少PM排放，但不一定会减少对健康的不利影响。
Debate about the biological effects of biodiesel exhaust emissions exists due to variation in methods of exhaust generation and biological models used to assess responses. Because studies in cells do not necessarily reflect the integrated response of a whole animal, experiments were conducted in two human cell lines representing bronchial epithelial cells and macrophages and female mice using identical particle suspensions of raw exhaust generated by a Volkswagen light-duty diesel engine using petrodiesel (B0) and a biodiesel blend (B20: 20% soy biodiesel/80% B0 by volume). Tailpipe particle emissions measurement showed B0 generated two times more particle mass, larger ultrafine particle number distribution modes, and particles of more nonpolar organic composition than the B20 fuel. Biological assays (inflammatory mediators, oxidative stress biomarkers) demonstrated that particulate matter (PM) generated by combustion of the two fuels induced different responses in in vitro and in vivo models. Concentrations of inflammatory mediators (Interleukin-6, IL-6; Interferon-gamma-induced Protein 10, IP-10; Granulocyte-stimulating factor, G-CSF) in the medium of B20-treated cells and in bronchoalveolar lavage fluid of mice exposed to B20 were ~20–30% higher than control or B0 PM, suggesting that addition of biodiesel to diesel fuels will reduce PM emissions but not necessarily adverse health outcomes.
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DOI：
10.1164/rccm.200611-1627oc
发表时间：
2007-08-15
影响因子：
24.7
作者：
Chuang, Kai-Jen;Chan, Chang-Chuan;Tang, Chin-Sheng
通讯作者：
Tang, Chin-Sheng
影响因子：
11.4
作者：
Biswas, Subhasis;Verma, Vishal;Sioutas, Constantinos
通讯作者：
Sioutas, Constantinos
影响因子：
10.4
作者：
Lewis JA;Rao KM;Castranova V;Vallyathan V;Dennis WE;Knechtges PL
通讯作者：
Knechtges PL
影响因子：
5.3
作者：
Knothe, Gerhard
通讯作者：
Knothe, Gerhard
影响因子：
2.1
作者：
Katre A;Ballinger C;Akhter H;Fanucchi M;Kim DK;Postlethwait E;Liu RM
通讯作者：
Liu RM