Detection of Excitatory and Inhibitory Synapses in the Auditory System Using Fluorescence Immunohistochemistry and High-Resolution Fluorescence Microscopy.
Detection of Excitatory and Inhibitory Synapses in the Auditory System Using Fluorescence Immunohistochemistry and High-Resolution Fluorescence Microscopy.
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使用荧光免疫组织化学和高分辨率荧光显微镜检测听觉系统中的兴奋性和抑制性突触
DOI：
10.1007/978-1-4939-3615-1_15
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发表时间：
2016
影响因子：
--
通讯作者：
Knipper M
中科院分区：
文献类型：
--
作者：
Singer W;Geisler HS;Panford-Walsh R;Knipper M
在感觉系统中，沿上升通路平衡的兴奋和抑制回路不仅对建立从外围到皮层的地形有序连接很重要，而且对信号处理的时间精度也很重要。在中枢神经系统中，空间和时间皮层分辨的完成是一个可能由第一次感觉体验启动的过程，它驱动了一段时间内皮层抑制的增加。在听觉系统中，初次感觉体验的时间也是耳蜗传出和传入纤维发生重组，导致内外毛细胞神经支配成熟的时期。这种成熟的毛细胞神经支配是准确的声音处理的基础，沿着上升通道向上到达听觉皮层。我们在这里描述了一种沿上行听觉通路检测兴奋性和抑制性标记蛋白的方案，这可能是检测各种形式听力障碍中听觉信号处理变化的有用工具。我们的方案使用荧光免疫组织化学结合高分辨率荧光显微镜在耳蜗和脑组织。
In sensory systems, a balanced excitatory and inhibitory circuit along the ascending pathway is not only important for the establishment of topographically ordered connections from the periphery to the cortex but also for temporal precision of signal processing. The accomplishment of spatial and temporal cortical resolution in the central nervous system is a process that is likely initiated by the first sensory experiences that drive a period of increased intracortical inhibition. In the auditory system, the time of first sensory experience is also the period in which a reorganization of cochlear efferent and afferent fibers occurs leading to the mature innervation of inner and outer hair cells. This mature hair cell innervation is the basis of accurate sound processing along the ascending pathway up to the auditory cortex. We describe here, a protocol for detecting excitatory and inhibitory marker proteins along the ascending auditory pathway, which could be a useful tool for detecting changes in auditory signal processing during various forms of hearing disorders. Our protocol uses fluorescence immunohistochemistry in combination with high-resolution fluorescence microscopy in cochlear and brain tissue.
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DOI：
10.1023/a:1024126110356
发表时间：
2002-03-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Kawaguchi, Y;Kondo, S
通讯作者：
Kondo, S
影响因子：
5.1
作者：
W. Singer;A. Zuccotti;Mirko Jaumann;S. C. Lee;R. Panford-Walsh;Hao Xiong;U. Zimmermann;Christoph Franz;Hyun-Soon Geisler;I. Köpschall;K. Rohbock;Ksenya Varakina;S. Verpoorten;T. Reinbothe;T. Schimmang;L. Rüttiger;M. Knipper
通讯作者：
W. Singer;A. Zuccotti;Mirko Jaumann;S. C. Lee;R. Panford-Walsh;Hao Xiong;U. Zimmermann;Christoph Franz;Hyun-Soon Geisler;I. Köpschall;K. Rohbock;Ksenya Varakina;S. Verpoorten;T. Reinbothe;T. Schimmang;L. Rüttiger;M. Knipper
交叉橄榄耳蜗束的电刺激对听觉敏感性临时阈值变化的影响。
影响因子：
2.5
作者：
R. Rajan
通讯作者：
R. Rajan
板下神经元消融改变神经营养蛋白表达和眼优势柱形成。
DOI：
10.1073/pnas.96.23.13491
发表时间：
1999
影响因子：
11.1
作者：
Lein,ES;Finney,EM;McQuillen,PS;Shatz,CJ
通讯作者：
Shatz,CJ
DOI：
10.1523/jneurosci.3974-06.2007
发表时间：
2007-03
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
Gaston Sendin;A. Bulankina;D. Riedel;T. Moser
通讯作者：
Gaston Sendin;A. Bulankina;D. Riedel;T. Moser