Transcriptomic analysis of cadmium stress response in the heavy metal hyperaccumulator Sedum alfredii Hance.
Transcriptomic analysis of cadmium stress response in the heavy metal hyperaccumulator Sedum alfredii Hance.
复制标题
重金属超富集植物景天镉胁迫反应的转录组分析
DOI：
10.1371/journal.pone.0064643
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu JX
中科院分区：
文献类型：
--
作者：
Gao J;Sun L;Yang X;Liu JX
东南景天超积累生态型(HE)具有在地上组织中超积累镉(Cd)、锌(Zn)和铅(Pb)的能力。虽然已经对这些植物进行了许多生理研究，但它们对重金属的高度耐受性的分子机制在很大程度上还不清楚。在这里，我们报道了利用罗氏454和Illumina/Solexa深度测序技术相结合的方法，在阿尔弗雷迪海棠(S.alfredii Hance(HE))芽中产生了9.4千兆碱基的接头剪裁的原始序列和57,162个转录重叠群的组装。我们还从功能上对转录组进行了注释，并分析了转录组在Cd超积累过程中的变化。在Q值为0.005的条件下，慢性Cd处理使阿尔弗雷迪海棠(S.alfredii Hance，HE)表达上调(Fold Change≧2.0)和下调(Fold Change≦0.5)的重叠群分别为110和12 3个。采用定量RT-PCR方法比较阿尔弗雷迪链霉菌(HE)和非超积累生态型(NHE)的基因表达谱。我们的结果表明，与细胞壁修饰、金属转移和再动员有关的几个基因在Cd胁迫下，在HE地上部比NHE地上部更高水平地诱导或结构性表达。总之，我们的研究提供了阿尔弗雷迪海棠(S.alfredii Hance(HE))嫩枝中CD反应的大规模表达序列信息和全基因组转录组谱。
The Sedum alfredii Hance hyperaccumulating ecotype (HE) has the ability to hyperaccumulate cadmium (Cd), as well as zinc (Zn) and lead (Pb) in above-ground tissues. Although many physiological studies have been conducted with these plants, the molecular mechanisms underlying their hyper-tolerance to heavy metals are largely unknown. Here we report on the generation of 9.4 gigabases of adaptor-trimmed raw sequences and the assembly of 57,162 transcript contigs in S. alfredii Hance (HE) shoots by the combination of Roche 454 and Illumina/Solexa deep sequencing technologies. We also have functionally annotated the transcriptome and analyzed the transcriptome changes upon Cd hyperaccumulation in S. alfredii Hance (HE) shoots. There are 110 contigs and 123 contigs that were up-regulated (Fold Change ≧2.0) and down-regulated (Fold Change ≦0.5) by chronic Cd treatment in S. alfredii Hance (HE) at q-value cutoff of 0.005, respectively. Quantitative RT-PCR was employed to compare gene expression patterns between S. alfredii Hance (HE) and non-hyperaccumulating ecotype (NHE). Our results demonstrated that several genes involved in cell wall modification, metal translocation and remobilization were more induced or constitutively expressed at higher levels in HE shoots than that in NHE shoots in response to Cd exposure. Together, our study provides large-scale expressed sequence information and genome-wide transcriptome profiling of Cd responses in S. alfredii Hance (HE) shoots.
登录
查看更多内容
影响因子：
6.9
作者：
Kaneda M;Schuetz M;Lin BS;Chanis C;Hamberger B;Western TL;Ehlting J;Samuels AL
通讯作者：
Samuels AL
影响因子：
5.8
作者：
Kofler, Robert;Schloetterer, Christian;Lelley, Tamas
通讯作者：
Lelley, Tamas
景天超富集生态型中镉从根到茎的易位增强
影响因子：
6.9
作者：
Lu, Ling-li;Tian, Sheng-ke;Yang, Xiao-e;Wang, Xiao-chang;Brown, Patrick;Li, Ting-qiang;He, Zhen-li
通讯作者：
He, Zhen-li
影响因子：
9.4
作者：
Tian, Sheng-Ke;Lu, Ling-Li;Brown, Patrick
通讯作者：
Brown, Patrick
影响因子：
6.9
作者：
Cai, Xiaoning;Davis, Elizabeth J.;Wu, Yajun
通讯作者：
Wu, Yajun