ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport.
ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport.
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DOI：
10.1093/jxb/erq416
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发表时间：
2011-03
影响因子：
6.9
通讯作者：
Samuels AL
中科院分区：
文献类型：
--
作者：
Kaneda M;Schuetz M;Lin BS;Chanis C;Hamberger B;Western TL;Ehlting J;Samuels AL
拟南芥的主花序茎在维管束和束间纤维中均富含木质素细胞壁。先前的基因表达研究表明苯丙素类生物合成基因的表达与编码ATP结合盒（ABC）转运蛋白的基因子集之间存在相关性，特别是在ABCB/多药耐药/P-糖蛋白（ABCB/MDR/PGP）和ABCG/多效性耐药（ABCG/PDR）亚家族中。本研究的目的是表征这些ABC转运蛋白在其基因表达和功能的发展中的脂细胞。基于计算机模拟分析，选择了4种ABC转运蛋白进行详细研究：ABCB 11/MDR 8、ABCB 14/MDR 12、ABCB 15/MDR 13和ABCG 33/PDR 5。每个基因的启动子：：葡萄糖醛酸酶报告基因分析表明ABCB 11、ABCB 14、ABCB 15和ABCG 33转运蛋白的启动子在初生茎的维管组织中有活性，在某些情况下在束间组织中也有活性。纯合T-DNA插入突变株系与野生型相比，没有表现出明显的不规则木质部表型或束间纤维木质化或形态学改变。然而，在abcb14 - 1突变体，茎维管形态略有混乱，减少韧皮部面积的维管束和木质部导管管腔直径减小。此外，abcb14 - 1突变体表现出减少极性生长素运输通过整个茎和改变生长素分布在原形成层。有人提出，ABCB14和ABCB15促进生长素运输，因为在这两个突变体的花序茎显示极性生长素运输减少，这是没有观察到任何测试的ABCG亚科突变体。在ABCB 14的情况下，生长素运输的减少与花序茎中脉管发育的轻度破坏相关。
The primary inflorescence stem of Arabidopsis thaliana is rich in lignified cell walls, in both vascular bundles and interfascicular fibres. Previous gene expression studies demonstrated a correlation between expression of phenylpropanoid biosynthetic genes and a subset of genes encoding ATP-binding cassette (ABC) transporters, especially in the ABCB/multi-drug resistance/P-glycoprotein (ABCB/MDR/PGP) and ABCG/pleiotropic drug resistance (ABCG/PDR) subfamilies. The objective of this study was to characterize these ABC transporters in terms of their gene expression and their function in development of lignified cells. Based on in silico analyses, four ABC transporters were selected for detailed investigation: ABCB11/MDR8, ABCB14/MDR12, ABCB15/MDR13, and ABCG33/PDR5. Promoter::glucuronidase reporter assays for each gene indicated that promoters of ABCB11, ABCB14, ABCB15, and ABCG33 transporters are active in the vascular tissues of primary stem, and in some cases in interfascicular tissues as well. Homozygous T-DNA insertion mutant lines showed no apparent irregular xylem phenotype or alterations in interfascicular fibre lignification or morphology in comparison with wild type. However, in abcb14-1 mutants, stem vascular morphology was slightly disorganized, with decreased phloem area in the vascular bundle and decreased xylem vessel lumen diameter. In addition, abcb14-1 mutants showed both decreased polar auxin transport through whole stems and altered auxin distribution in the procambium. It is proposed that both ABCB14 and ABCB15 promote auxin transport since inflorescence stems in both mutants showed a reduction in polar auxin transport, which was not observed for any of the ABCG subfamily mutants tested. In the case of ABCB14, the reduction in auxin transport is correlated with a mild disruption of vascular development in the inflorescence stem.
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DOI：
10.1073/pnas.0506958103
发表时间：
2006-04-25
影响因子：
11.1
作者：
Dunkley, TPJ;Hester, S;Lilley, KS
通讯作者：
Lilley, KS
影响因子：
9.2
作者：
Heisler, MG;Ohno, C;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
11.6
作者：
Jasinski, M;Stukkens, Y;Boutry, M
通讯作者：
Boutry, M
影响因子：
4.6
作者：
Mravec, Jozef;Kubes, Martin;Friml, Jiri
通讯作者：
Friml, Jiri
影响因子：
7.4
作者：
Murphy, AS;Hoogner, KR;Taiz, L
通讯作者：
Taiz, L