Uncovering cryptic diversity in Aspidomorphus (Serpentes: Elapidae): evidence from mitochondrial and nuclear markers.

Uncovering cryptic diversity in Aspidomorphus (Serpentes: Elapidae): evidence from mitochondrial and nuclear markers.
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揭示 Aspidomorphus(蛇类:Elapidae)的神秘多样性:来自线粒体和核标记的证据。

DOI:
10.1016/j.ympev.2009.07.027
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发表时间:
2010
影响因子:
4.1
通讯作者:
Parkinson,ChristopherL
Parkinson,ChristopherL
中科院分区:
生物学1区
文献类型:
--
作者:
Metzger,GenevieveA;Kraus,Fred;Allison,Allen;Parkinson,ChristopherL

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巴布亚地区由新几内亚和附近岛屿组成,有着复杂的地质历史,孕育了高水平的生物多样性和特有现象。不幸的是,这种多样性的大部分仍然没有记录。我们研究了巴布亚特有毒蛇 Aspidomorphus 属(Elapidae:Hydrophiinae)的进化关系,并记录了广泛的神秘谱系多样性。在 Aspidomorphus 物种之间,我们发现 22.2-27.9% 的校正 cyt-b 序列差异。在物种内,我们发现最大序列分歧为 17.7–23.7%。这些高水平的遗传差异可能使之前的系统发育研究变得复杂,这些研究很难将 Aspidomorphus 归入 Hydrophiinae 亚科。与以前的研究相比,我们增加了 Hydrophiinae 内的采样,以包括所有目前已识别的 Aspidomorphus 物种,并增加了 Demansia 属和 Toxicocalamus 属的物种代表性。我们利用一组七个分子标记(12S、16S、cyt-b、ND4、c-mos、MyHC-2 和 RAG-1)确认了 Aspidomorphus 的单系性并解决了该属的放置;我们发现强有力的证据支持 Aspidomorphus 和 Demansia/Toxicocalamus preussi 分支之间存在姐妹群关系。我们还使用一种线粒体 (cyt-b) 和一种核标记 (SPTBN1) 来记录所有目前公认的 Aspidomorphus 物种内的深层遗传分化,并讨论所罗门岛弧作为该物种潜在的分化中心。最后,我们发现用于种间系统发育重建的 mtDNA 和 nuDNA 标记之间具有高度的一致性。
The Papuan region, comprising New Guinea and nearby islands, has a complex geological history that has fostered high levels of biodiversity and endemism. Unfortunately, much of this diversity remains undocumented. We examine the evolutionary relationships of the venomous snake genus Aspidomorphus (Elapidae: Hydrophiinae), a Papuan endemic, and document extensive cryptic lineage diversification. Between Aspidomorphus species we find 22.2–27.9% corrected cyt-b sequence divergence. Within species we find 17.7–23.7% maximum sequence divergence. These high levels of genetic divergence may have complicated previous phylogenetic studies, which have had difficulty placing Aspidomorphus within the subfamily Hydrophiinae. Compared to previous studies, we increase sampling within Hydrophiinae to include all currently recognized species of Aspidomorphus and increase species representation for the genera Demansia and Toxicocalamus. We confirm monophyly of Aspidomorphus and resolve placement of the genus utilizing a set of seven molecular markers (12S, 16S, cyt-b, ND4, c-mos, MyHC-2, and RAG-1); we find strong support for a sister-group relationship between Aspidomorphus and a Demansia/Toxicocalamus preussi clade. We also use one mitochondrial (cyt-b) and one nuclear marker (SPTBN1) to document deep genetic divergence within all currently recognized species of Aspidomorphus and discuss the Solomon Island Arc as a potential center of divergence in this species. Lastly, we find high levels of concordance between the mtDNA and nuDNA markers used for inter-species phylogenetic reconstruction.
基于细胞色素 b 和 16S rRNA 序列的陆地澳大利亚-巴布亚蛇类(Hydrophiinae 亚科)的系统发育关系。
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