Hydrogen peroxide modulates energy metabolism and oxidative stress in cultures of permanent human Müller cells MIO‐M1
Hydrogen peroxide modulates energy metabolism and oxidative stress in cultures of permanent human Müller cells MIO‐M1
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过氧化氢调节永久性人类 Müller 细胞 MIOâM1 培养物中的能量代谢和氧化应激
DOI：
10.1002/jbio.201600201
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发表时间：
2016
影响因子：
2.8
通讯作者：
M. Hammer
中科院分区：
文献类型：
--
作者：
S. Peters;M. Griebsch;M. Klemm;J. Haueisen;M. Hammer
在这项研究中，过氧化氢（H2 O2）的氧化还原状态，NADH蛋白结合，和线粒体膜电位在Müller细胞的影响进行了研究。将永久性人Müller细胞MIO-M1的培养物暴露于75 µM和150 µM浓度的H2 O2中2小时。通过双光子显微镜（激发波长：740 nm）测量线粒体的荧光发射光谱和寿命，在显微图像中通过其荧光性质（光谱和强度）识别线粒体。H2 O2暴露2小时不会损害培养物中MIO-M1细胞的活力。然而，在任一H2 O2浓度下，黄素与NADH荧光强度的比值均未发生变化，未暴露于H2 O2的对照组与暴露于150 µM H2 O2的对照组之间的平均寿命存在显著差异（972 ± 63 ps vs. 1152 ± 64 ps，p= 0.014）。停止H_2O_2暴露后1小时，其值恢复到对照组的值（983 ± 36 ps）。在150 μM H2 O2下发现线粒体膜的超极化。这些发现表明线粒体中游离的NADH转变为蛋白结合的NADH，以及线粒体内膜的超极化，这可能与Müller细胞功能受损有关，尽管它们保持活力。将人Müller细胞暴露于过氧化氢2小时导致线粒体NADH蛋白结合的可逆变化在氧化还原比不变的情况下。线粒体膜电位在暴露过程中增加。
In this study the influence of hydrogen peroxide (H2O2) on the redox state, NADH protein binding, and mitochondrial membrane potential in Müller cells is investigated. Cultures of permanent human Müller cells MIO‐M1 were exposed to H2O2in 75 µM and 150 µM concentration for two hours. Fluorescence emission spectra and lifetimes were measured by two‐photon microscopy (excitation wavelength: 740 nm) at the mitochondria which were identified in the microscopic images by their fluorescence properties (spectra and intensity). Two hours of H2O2exposure did not impair viability of MIO‐M1 cells in culture. Whereas the ratio of flavine‐ to NADH fluorescence intensity did not change under either H2O2concentration, the mean lifetime was significantly different between controls, not exposed to H2O2, and the 150 µM H2O2exposure (972 ± 63 ps vs. 1152 ± 64 ps,p= 0.014). One hour after cessation of the H2O2exposure, the value retuned to that of the control (983 ± 36 ps). A hyperpolarization of the mitochondrial membrane under 150 µM H2O2was found. These findings suggest a shift form free to protein‐bound NADH in mitochondria as well as a hyperpolarization of their inner membrane which could be related to an impairment of Müller cell function despite their preserved viability.Exposure of human Müller cells to hydrogen peroxide for two hours results in a reversible change of protein binding of mitochondrial NADH upon unchanged redox ratio. The mitochondrial membrane potential is increased during exposure.
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影响因子：
2.2
作者：
Heikal AA
通讯作者：
Heikal AA
影响因子：
15.9
作者：
Harada, Takayuki;Harada, Chikako;Tanaka, Kohichi
通讯作者：
Tanaka, Kohichi
影响因子：
3.7
作者：
Klemm M;Schweitzer D;Peters S;Sauer L;Hammer M;Haueisen J
通讯作者：
Haueisen J
缺血再灌注模型中兔 Müller 细胞中热休克蛋白 70 表达增加
影响因子：
2.1
作者：
T. Gohdo;H. Ueda;S. Ohno;H. Iijima;S. Tsukahara
通讯作者：
S. Tsukahara
聚（ADP-核糖）聚合酶的抑制可在过氧化氢暴露后保留表面活性剂的合成。
DOI：
10.1152/ajplung.1995.269.1.l59
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
Hudak,BB;Tufariello,J;Sokolowski,J;Maloney,C;Holm,BA
通讯作者：
Holm,BA