Enzyme immobilization: an overview on techniques and support materials.

Enzyme immobilization: an overview on techniques and support materials.
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DOI:
10.1007/s13205-012-0071-7
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发表时间:
2013-02
期刊:
影响因子:
2.8
通讯作者:
Rajaram, Yamuna Rani Sriramulu
Rajaram, Yamuna Rani Sriramulu
中科院分区:
工程技术4区
文献类型:
--
作者:
Datta, Sumitra;Christena, L. Rene;Rajaram, Yamuna Rani Sriramulu

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目前世界生物技术工业的需求是提高酶的生产率和开发新技术以延长其保质期。这些要求是不可避免的,以促进大规模和经济的配制。酶的固定化提供了一个很好的基础,增加酶的可用性,以更大的营业额在相当长的时间内的底物。已经评估了几种天然和合成支持物用于酶固定化的效率。如今,固定化酶比它们的游离对应物更受欢迎,因为它们的长期可用性减少了冗余的下游和纯化过程。未来的研究应奋进采用逻辑和合理的包埋技术沿着与创新的改性支持,以改善酶的固定化状态,并提供新的前景,工业部门。
The current demands of the world’s biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. These requirements are inevitable to facilitate large-scale and economic formulation. Enzyme immobilization provides an excellent base for increasing availability of enzyme to the substrate with greater turnover over a considerable period of time. Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization. Nowadays, immobilized enzymes are preferred over their free counterpart due to their prolonged availability that curtails redundant downstream and purification processes. Future investigations should endeavor at adopting logistic and sensible entrapment techniques along with innovatively modified supports to improve the state of enzyme immobilization and provide new perspectives to the industrial sector.
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