Molecular verification of commercialized Neoseiulus californicus (McGregor) settlement before spider mite appearance in a Japanese pear orchard

Molecular verification of commercialized Neoseiulus californicus (McGregor) settlement before spider mite appearance in a Japanese pear orchard
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日本梨园红蜘蛛出现前商业化的加州新绥螨(McGregor)定居点的分子验证

DOI:
10.11158/saa.27.12.3
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发表时间:
2022
影响因子:
1.2
通讯作者:
Shoji Sonoda
Shoji Sonoda
中科院分区:
农林科学3区
文献类型:
--
作者:
Yuya Mikawa;Mineaki Aizawa;Kotaro Mori;Masatoshi Toyama;Shoji Sonoda

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我们早期研究的一份报告描述了一种植绥螨加利福尼亚新绥螨(McGregor)(阿卡里:植绥螨科)释放系统,使用保湿保护袋控制叶螨。在本研究中,我们在日本果园的71棵树中的43棵上安装了捕食者释放系统,以调查N的扩散。加利福尼亚州从捕食者释放系统释放。在果园里,日本梨树被训练在高架水平棚架系统上。因此,这些树通过网格训练系统相互连接。我们收集植绥螨的树叶,并进行PCR检测其物种组成。结果表明,N.加利福尼亚州占62.37%。利用微卫星标记,商业化的N.从捕食者释放系统释放的加利福尼亚人与土著人不同。加利福尼亚州。结果表明,植绥螨主要由商品化N.加利福尼亚州发生一个星期前,在树木与捕食者释放系统。结果还表明,商品化的N.加利福尼亚州的扩散主要发生在安装捕食者释放系统的树木中。
A report of our earlier study described a phytoseiid miteNeoseiulus californicus(McGregor) (Acari: Phytoseiidae) release system using a moisturized sheltered sachet for spider mite control. For the present study, we installed the predator release systems on 43 out of 71 trees in a Japanese pear orchard to survey the dispersal ofN. californicusreleased from the predator release system. In the orchard, Japanese pear trees were trained on the overhead horizontal trellis system. Consequently, the trees were mutually connected through the trellis training system. We collected phytoseiid mites on the tree leaves and were subjected to PCR to examine their species composition. Results demonstrated thatN. californicuswere 62.37% of the total of 3,247 phytoseiid mites collected. Using microsatellite markers, commercializedN. californicusthat had been released from the predator release systems were discriminated from indigenousN. californicus. Results demonstrated that phytoseiid mites consisting mainly of the commercializedN. californicusoccurred one week earlier in trees with the predator release systems. Results also demonstrated that the commercializedN. californicusdispersal occurred mainly in trees installed with the predator release systems.
法国南部果园秋季、冬季和春季期间加州新螨(螨虫:植螨科)的位置、繁殖和活动
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