Cell type specific accumulation of the major latency-associated transcript (LAT) of herpes simplex virus type 2 in LAT transgenic mice.
Cell type specific accumulation of the major latency-associated transcript (LAT) of herpes simplex virus type 2 in LAT transgenic mice.
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DOI：
10.1016/j.virol.2008.12.035
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发表时间：
2009-03-30
期刊：
影响因子：
3.7
通讯作者：
Cohen, Jeffrey I.
中科院分区：
文献类型：
--
作者：
Wang, Kening;Mahalingam, Gowtham;Imai, Yumi;Pesnicak, Lesley;Margolis, Todd T.;Straus, Stephen E.;Cohen, Jeffrey I.
关键词：
我们进行了原位杂交，以确定潜伏相关转录本（LAT）的内含子在HSV-2 LAT转基因小鼠组织中的细胞类型特异性积累，其中LAT表达由其天然启动子驱动。我们在从HSV-2 LAT转基因小鼠分析的大多数组织中的多种细胞类型中鉴定了LAT。虽然在脑和脊髓神经元、上皮细胞和肌肉细胞中观察到弱至中等信号，但在背根和三叉神经节的神经元中检测到最强的信号。这些神经节中约70-86%的神经元呈不同强度的LAT阳性，而神经元周围的细胞呈LAT阴性。LAT阳性神经元和总神经元中A5或KH 10阳性神经元的频率相似。这些数据表明HSV-2 LAT启动子活性不限于神经元，并且LAT在神经节神经元中的积累可能受细胞特异性因子调节。
We performed in situ hybridization to determine the cell type specific accumulation of the intron of the latency-associated transcript (LAT) in tissues in HSV-2 LAT transgenic mice in which LAT expression is driven by its native promoter. We identified LAT in multiple cell types in most tissues analyzed from HSV-2 LAT transgenic mice. While weak to moderate signals were seen in brain and spinal cord neurons, epithelial cells, and muscle cells, the strongest signals were detected in neurons from dorsal root and trigeminal ganglia. About 70–86% of neurons in these ganglia were LAT-positive with varying signal intensities, while cells surrounding the neurons were LAT-negative. The frequency of A5 or KH10-positive neurons was similar in LAT-positive and total neurons. These data indicate that HSV-2 LAT promoter activity is not restricted to neurons and that LAT accumulation in ganglionic neurons is likely regulated by cell-specific factors.
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影响因子：
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作者：
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通讯作者：
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DOI：
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作者：
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