Raman microscopy for noninvasive imaging of pharmaceutical nanocarriers: intracellular distribution of cationic liposomes of different composition.
Raman microscopy for noninvasive imaging of pharmaceutical nanocarriers: intracellular distribution of cationic liposomes of different composition.
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DOI：
10.1021/mp200519y
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发表时间：
2012-04-02
影响因子：
4.9
通讯作者：
Torchilin V
中科院分区：
文献类型：
--
作者：
Chernenko T;Sawant RR;Miljkovic M;Quintero L;Diem M;Torchilin V
Nanotechnology is playing an increasing role in targeted drug delivery into pathological tissues. Drug-loaded pharmaceutical nanocarriers can be delivered into diseased sites by passive targeting (spontaneous accumulation of nanocarriers in the areas with affected vasculature) or by active targeting (via site-specific ligands attached to the surface of drug-loaded nanocarriers). Subsequent level of targeting requires cellular internalization of nanocarriers and their specific association with certain individual cell organelles. The control over intracellular distribution of pharmaceutical nanocarriers requires effective and non-invasive methods of their visualization inside cells. In an attempt to enhance cellular internalization of pharmaceutical nanocarriers and their association with mitochondria specifically, we have prepared three types of cationic liposomes and investigated their intracellular distribution. The analysis was performed using Raman microspectroscopy in combination with optical microscopy, in order to provide morphological information as well as biochemical signatures of the sample. It was demonstrated that the Raman microscopy allows to evaluate the extent of mitochondrial association depending on the liposome composition.
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通讯作者：
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