Morphological plasticity promotes resistance to phagocyte killing of uropathogenic Escherichia coli.
Morphological plasticity promotes resistance to phagocyte killing of uropathogenic Escherichia coli.
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DOI：
10.1016/j.micinf.2010.12.004
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发表时间：
2011-05
影响因子：
5.8
通讯作者：
Justice SS
中科院分区：
文献类型：
--
作者：
Horvath DJ Jr;Li B;Casper T;Partida-Sanchez S;Hunstad DA;Hultgren SJ;Justice SS
尿路致病性大肠杆菌通过复杂的细胞内发育途径进行，包括多种形态变化。在toll样受体4激活的浅表膀胱上皮细胞的细胞内生长过程中，尿路致病性大肠杆菌亚群启动苏拉介导的丝化。在这项研究中，我们直接研究了细菌形态在尿路致病性大肠杆菌被吞噬细胞杀死后存活的作用。我们最初确定，多形核中性粒细胞和巨噬细胞在与细胞外细菌和丝状尿路致病性大肠杆菌同时被募集到小鼠膀胱上皮。我们进一步确定，当体外培养的小鼠巨噬细胞攻击混合的尿路致病性大肠杆菌时，细菌性尿路致病性大肠杆菌优先被破坏。与使用椭圆形状聚合物的研究一致，吞噬细胞与丝状尿路致病性大肠杆菌之间的初始接触点影响了内化的效果。这些发现表明，丝状形态为尿路致病性大肠杆菌逃避吞噬细胞的杀伤提供了选择优势，并确定了SulA在细菌性膀胱炎中的重要作用机制。因此，形态可塑性可以被视为细菌病原体用来破坏宿主免疫的一种独特的机制。
Uropathogenic Escherichia coli proceed through a complex intracellular developmental pathway that includes multiple morphological changes. During intracellular growth within Toll-like receptor 4-activated superficial bladder epithelial cells, a subpopulation of uropathogenic E. coli initiates SulA-mediated filamentation. In this study, we directly investigated the role of bacterial morphology in the survival of uropathogenic E. coli from killing by phagocytes. We initially determined that both polymorphonuclear neutrophils and macrophages are recruited to murine bladder epithelium at times coincident with extracellular bacillary and filamentous uropathogenic E. coli. We further determined that bacillary uropathogenic E. coli were preferentially destroyed when mixed uropathogenic E. coli populations were challenged with cultured murine macrophages in vitro. Consistent with studies using elliptical-shaped polymers, the initial point of contact between the phagocyte and filamentous uropathogenic E. coli influenced the efficacy of internalization. These findings demonstrate that filamentous morphology provides a selective advantage for uropathogenic E. coli evasion of killing by phagocytes and defines a mechanism for the essential role for SulA during bacterial cystitis. Thus, morphological plasticity can be viewed as a distinct class of mechanism used by bacterial pathogens to subvert host immunity.
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DOI：
10.1084/jem.20031237
发表时间：
2004-01-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Doyle SE;O'Connell RM;Miranda GA;Vaidya SA;Chow EK;Liu PT;Suzuki S;Suzuki N;Modlin RL;Yeh WC;Lane TF;Cheng G
通讯作者：
Cheng G
影响因子：
3.1
作者：
Leroy, Magali;Cabral, Howard;Goldstein, Richard
通讯作者：
Goldstein, Richard
影响因子：
15.9
作者：
AGACE, WW;HEDGES, SR;SVANBORG, C
通讯作者：
SVANBORG, C
影响因子：
3.4
作者：
Ingersoll MA;Kline KA;Nielsen HV;Hultgren SJ
通讯作者：
Hultgren SJ
影响因子：
3.1
作者：
Mulvey, MA;Schilling, JD;Hultgren, SJ
通讯作者：
Hultgren, SJ