Synthetic genomics: a new venture to dissect genome fundamentals and engineer new functions.
Synthetic genomics: a new venture to dissect genome fundamentals and engineer new functions.
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DOI：
10.1016/j.cbpa.2018.04.002
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发表时间：
2018-10
影响因子：
7.8
通讯作者：
Cai Y
中科院分区：
文献类型：
--
作者：
Schindler D;Dai J;Cai Y
病毒、细胞器和细菌的基因组可以合成。Sc2.0正在产生第一个合成的设计师真核生物。17 S中的6酿酒酵母染色体被合成并发表。GP-write将开启合成基因组学的新时代。自20世纪70年代第一个人工合成基因问世以来，DNA序列定制合成的效率和能力已经提高了几个数量级。在过去几年中，DNA合成和组装的进步导致定制DNA的价格急剧下降。在DNA测序技术中也观察到了类似的效果，DNA测序技术是DNA阅读项目的基础。今天，具有超过10 000 bp和几周的周转时间的合成DNA序列是商业上可获得的。这使研究人员能够执行大规模项目来编写合成染色体并在体内表征其功能。合成基因组学开辟了研究基因组基本原理和设计新生物功能的新范式。
Viral, organelle and bacterial genomes can be synthesized. Sc2.0 is on the way to generating the first synthetic designer eukaryote. 6 of 17 S. cerevisiae chromosomes are synthesized and published. GP-write will open a new era of synthetic genomics. Since the first synthetic gene was synthesized in 1970s, the efficiency and the capacity of made-to-order DNA sequence synthesis has increased by several orders of magnitude. Advances in DNA synthesis and assembly over the past years has resulted in a steep drop in price for custom made DNA. Similar effects were observed in DNA sequencing technologies which underpin DNA-reading projects. Today, synthetic DNA sequences with more than 10 000 bps and turn-around times of a few weeks are commercially available. This enables researchers to perform large-scale projects to write synthetic chromosomes and characterize their functionalities in vivo. Synthetic genomics opens up new paradigms to study the genome fundamentals and engineer novel biological functions.
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影响因子：
6.7
作者：
Lee YN;Bieniasz PD
通讯作者：
Bieniasz PD
影响因子：
56.9
作者：
Richardson, Sarah M.;Mitchell, Leslie A.;Bader, Joel S.
通讯作者：
Bader, Joel S.
影响因子：
64.8
作者：
Dymond, Jessica S.;Richardson, Sarah M.;Coombes, Candice E.;Babatz, Timothy;Muller, Heloise;Annaluru, Narayana;Blake, William J.;Schwerzmann, Joy W.;Dai, Junbiao;Lindstrom, Derek L.;Boeke, Annabel C.;Gottschling, Daniel E.;Chandrasegaran, Srinivasan;Bader, Joel S.;Boeke, Jef D.
通讯作者：
Boeke, Jef D.
DOI：
10.1126/science.1249252
发表时间：
2014-04-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Annaluru N;Muller H;Mitchell LA;Ramalingam S;Stracquadanio G;Richardson SM;Dymond JS;Kuang Z;Scheifele LZ;Cooper EM;Cai Y;Zeller K;Agmon N;Han JS;Hadjithomas M;Tullman J;Caravelli K;Cirelli K;Guo Z;London V;Yeluru A;Murugan S;Kandavelou K;Agier N;Fischer G;Yang K;Martin JA;Bilgel M;Bohutski P;Boulier KM;Capaldo BJ;Chang J;Charoen K;Choi WJ;Deng P;DiCarlo JE;Doong J;Dunn J;Feinberg JI;Fernandez C;Floria CE;Gladowski D;Hadidi P;Ishizuka I;Jabbari J;Lau CY;Lee PA;Li S;Lin D;Linder ME;Ling J;Liu J;Liu J;London M;Ma H;Mao J;McDade JE;McMillan A;Moore AM;Oh WC;Ouyang Y;Patel R;Paul M;Paulsen LC;Qiu J;Rhee A;Rubashkin MG;Soh IY;Sotuyo NE;Srinivas V;Suarez A;Wong A;Wong R;Xie WR;Xu Y;Yu AT;Koszul R;Bader JS;Boeke JD;Chandrasegaran S
通讯作者：
Chandrasegaran S
DOI：
10.1073/pnas.1110889108
发表时间：
2011-10-18
影响因子：
11.1
作者：
Arslan, Defne;Legendre, Matthieu;Claverie, Jean-Michel
通讯作者：
Claverie, Jean-Michel