Sperm-oocyte contact induces outside-in signaling via PYK2 activation.
Sperm-oocyte contact induces outside-in signaling via PYK2 activation.
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DOI：
10.1016/j.ydbio.2017.05.016
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发表时间：
2017-08-01
影响因子：
2.7
通讯作者：
Kinsey WH
中科院分区：
文献类型：
--
作者：
Wang H;Luo J;Carlton C;McGinnis LK;Kinsey WH
受精是一个多步骤的过程，开始于质膜相互作用，使精子-卵母细胞结合，然后是精子和卵母细胞质膜的融合。一旦发生膜融合，精子结合涉及卵母细胞皮质内的肌动蛋白重塑事件，允许精子头部穿透皮质肌动蛋白层并进入卵质。尽管对生殖的重要性，配子结合，融合和精子结合的控制机制知之甚少。虽然已知富含脯氨酸的酪氨酸激酶2（PYK 2或PTK 2b）激酶活性在受精中起重要作用，但其特定功能尚未得到解决。本研究利用小鼠卵母细胞去透明带受精试验来研究PYK 2活性与精子-卵母细胞结合和融合之间的关系，以及肌动蛋白聚合和精子掺入的局部变化。在该试验中，大多数结合的精子对卵母细胞没有明显的影响，只有少数结合到卵质中。然而，结合精子的一个子集与PYK 2被招募到卵母细胞皮层的局部反应相关，在那里它经常与f-肌动蛋白的环或盘共定位。在pyk 2 −/−卵母细胞中，表现出这种肌动蛋白反应的精子-卵母细胞结合位点的频率降低，并且pyk 2 −/−卵母细胞在整合精子方面的效率较低，表明这种蛋白激酶可能在精子整合中起重要作用。PYK 2对精子-卵母细胞相互作用的反应似乎与配子融合无关，因为PYK 2在精子-卵母细胞融合被阻止的条件下被募集到精子结合位点，并且因为PYK 2抑制或消除不阻止精子-卵母细胞融合。虽然卵母细胞中PYK 2反应与单个结合精子的成功结合之间的直接相关性仍有待建立，但这些发现表明了一种模型，其中卵母细胞不是受精的被动参与者，而是通过局部PYK 2信号传导对精子接触做出反应，该信号传导促进将精子头部物理结合到卵质中所需的肌动蛋白重塑事件。
Fertilization is a multi-step process that begins with plasma membrane interactions that enable sperm - oocyte binding followed by fusion of the sperm and oocyte plasma membranes. Once membrane fusion has occurred, sperm incorporation involves actin remodeling events within the oocyte cortex that allow the sperm head to penetrate the cortical actin layer and gain access to the ooplasm. Despite the significance for reproduction, the control mechanisms involved in gamete binding, fusion, and sperm incorporation are poorly understood. While it is known that proline - rich tyrosine kinase 2 (PYK2 or PTK2b) kinase activity plays an important role in fertilization, its specific function has not been addressed. The present study made use of a zona-free mouse oocyte fertilization assay to investigate the relationship between PYK2 activity and sperm - oocyte binding and fusion, as well as localized changes in actin polymerization and sperm incorporation. In this assay, the majority of bound sperm had no apparent effect on the oocyte and only a few became incorporated into the ooplasm. However, a subset of bound sperm were associated with a localized response in which PYK2 was recruited to the oocyte cortex where it frequently co-localized with a ring or disk of f-actin. The frequency of sperm-oocyte binding sites that exhibited this actin response was reduced in pyk2−/− oocytes and the pyk2−/− oocytes proved less efficient at incorporating sperm, indicating that this protein kinase may have an important role in sperm incorporation. The response of PYK2 to sperm-oocyte interaction appeared unrelated to gamete fusion since PYK2 was recruited to sperm - binding sites under conditions where sperm - oocyte fusion was prevented and since PYK2 suppression or ablation did not prevent sperm - oocyte fusion. While a direct correlation between the PYK2 response in the oocyte and the successful incorporation of individual bound sperm remains to be established, these findings suggest a model in which the oocyte is not a passive participant in fertilization, but instead responds to sperm contact by localized PYK2 signaling that promotes actin remodeling events required to physically incorporate the sperm head into the ooplasm.
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DOI：
10.1523/jneurosci.4992-08.2010
发表时间：
2010-01-13
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Bartos JA;Ulrich JD;Li H;Beazely MA;Chen Y;Macdonald JF;Hell JW
通讯作者：
Hell JW
DOI：
10.1155/2012/296450
发表时间：
2012
期刊：
Journal of signal transduction
影响因子：
--
作者：
Eleniste PP;Bruzzaniti A
通讯作者：
Bruzzaniti A
影响因子：
2.5
作者：
McGinnis, Lynda K.;Luo, Jinping;Kinsey, William H.
通讯作者：
Kinsey, William H.
DOI：
10.1002/mrd.1120140402
发表时间：
1986-01-01
期刊：
GAMETE RESEARCH
影响因子：
--
作者：
CLINE, CA;SCHATTEN, G
通讯作者：
SCHATTEN, G
DOI：
10.1002/mrd.1120210207
发表时间：
1988-10-01
期刊：
GAMETE RESEARCH
影响因子：
--
作者：
KYOZUKA, K;OSANAI, K
通讯作者：
OSANAI, K