Histochemical demonstration of a monocarboxylate transporter in the mouse perineurium with special reference to GLUT1.
Histochemical demonstration of a monocarboxylate transporter in the mouse perineurium with special reference to GLUT1.
复制标题
小鼠神经束膜中单羧酸转运蛋白的组织化学演示，特别参考 GLUT1。
DOI：
10.2220/biomedres.29.297
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
T. Iwanaga
中科院分区：
文献类型：
--
作者：
Kumiko Takebe;J. Nio;H. Takahashi;T. Iwanaga
周围神经在神经内膜血管和神经束膜中都表达GLUT 1，并利用葡萄糖作为主要的能量底物，大脑也是如此。然而，在葡萄糖利用减少的条件下，大脑依赖于单羧酸盐，如酮体和乳酸盐，伴随着血脑屏障中单羧酸转运蛋白（MCT 1）的表达升高。本免疫组织化学研究旨在检查MCT 1在小鼠周围神经中的表达。MCT 1免疫反应性被发现在神经束膜鞘和共定位与GLUT 1，而神经内膜血管只表达GLUT 1。免疫印迹和原位杂交分析证实了MCT 1在神经束膜中的强烈表达。在超微结构上，MCT1和GLUT1的免疫反应在厚的神经束膜呈梯度下降，向最内层。在新生儿中，神经束膜中的MCT 1免疫反应强烈，而GLUT 1免疫反应微弱或不存在。这些研究结果表明，周围神经依赖于单羧酸作为一个主要的能量来源，并在神经束膜中的MCT 1是负责供应的单羧酸的神经纤维和雪旺细胞。
Peripheral nerves express GLUT1 in both endoneurial blood vessels and the perineurium and utilize glucose as a major energy substrate, as does the brain. However, under conditions of a reduced utilization of glucose, the brain is dependent upon monocarboxylates such as ketone bodies and lactate, being accompanied by an elevated expression of a monocarboxylate transporter (MCT1) in the blood-brain barrier. The present immunohistochemical study aimed to examine the expression of MCT1 in the peripheral nerves of mice. MCT1 immunoreactivity was found in the perineurial sheath and colocalized with GLUT1, while the endoneurial blood vessels expressed GLUT1 only. An intense expression of MCT1 in the perineurium was confirmed by Western blot and in situ hybridization analyses. Ultrastructurally, the MCT1 and GLUT1 immunoreactivities in the thick perineurium showed an intensity gradient decreasing towards the innermost layer. In neonates, the MCT1 immunoreactivity in the perineurium was intense, while the GLUT1 immunoreactivity was faint or absent. These findings suggest that peripheral nerves depend on monocarboxylates as a major energy source and that MCT1 in the perineurium is responsible for the supply of monocarboxylates to nerve fibers and Schwann cells.
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DOI：
10.1152/ajpendo.1997.273.1.e207
发表时间：
1997-07-01
影响因子：
5.1
作者：
Gerhart, DZ;Enerson, BE;Drewes, LR
通讯作者：
Drewes, LR
DOI：
10.1152/ajpendo.1998.275.3.e516
发表时间：
1998-09-01
影响因子：
5.1
作者：
Koehler-Stec, EM;Simpson, IA;Landschulz, WH
通讯作者：
Landschulz, WH
DOI：
10.1016/s0165-3806(98)00188-6
发表时间：
1999-03-12
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Leino, RL;Gerhart, DZ;Drewes, LR
通讯作者：
Drewes, LR
DOI：
10.1152/ajpendo.00187.2003
发表时间：
2003-11-01
影响因子：
5.1
作者：
Vannucci, SJ;Simpson, IA
通讯作者：
Simpson, IA