PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis.
PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis.
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DOI：
10.1083/jcb.201106093
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发表时间：
2011-12-26
期刊：
影响因子：
--
通讯作者：
Rhee K
中科院分区：
文献类型：
--
作者：
Lee K;Rhee K
plk1介导的心周蛋白磷酸化诱导梭形极特异性心周基质的适当组织和随后的中心体成熟。中心体的微管组织活性在细胞周期中振荡，在有丝分裂时达到最高水平。在有丝分裂开始时，中心体经历成熟，其特征是中心周围基质（PCM）的急剧扩张和微管组织活性的强劲增加。众所周知，PLK1对于中心体成熟的起始至关重要。在本文中，我们报道了一种PCM蛋白PCNT在有丝分裂过程中被PLK1特异性磷酸化。PCNT的耐磷点突变体在有丝分裂过程中不招募中心体蛋白，如CEP192、GCP-WD （γ-complex protein with WD repeats）、γ-微管蛋白、Aurora A和PLK1进入中心体。然而，CEP215的中心体募集依赖于PCNT，而不管其磷酸化状态如何。此外，PLK1-PCNT融合蛋白的异位表达甚至在间期细胞中诱导CEP192、GCP-WD和γ-微管蛋白的中心体积累，模拟中心体成熟。基于这些结果，我们提出plk1介导的PCNT磷酸化通过组织纺锤极特异性PCM晶格来启动中心体成熟。
PLK1-mediated phosphorylation of pericentrin induces proper organization of the spindle pole–specific pericentriolar matrix and subsequent centrosome maturation. The microtubule-organizing activity of the centrosome oscillates during the cell cycle, reaching its highest level at mitosis. At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar matrix (PCM) and a robust increase in microtubule-organizing activity. It is known that PLK1 is critical for the initiation of centrosome maturation. In this paper, we report that pericentrin (PCNT), a PCM protein, was specifically phosphorylated by PLK1 during mitosis. Phosphoresistant point mutants of PCNT did not recruit centrosomal proteins, such as CEP192, GCP-WD (γ-complex protein with WD repeats), γ-tubulin, Aurora A, and PLK1, into the centrosome during mitosis. However, centrosomal recruitment of CEP215 depended on PCNT irrespective of its phosphorylation status. Furthermore, ectopic expression of PLK1-PCNT fusion proteins induced the centrosomal accumulation of CEP192, GCP-WD, and γ-tubulin even in interphase cells, mimicking centrosome maturation. Based on these results, we propose that PLK1-mediated phosphorylation of PCNT initiates centrosome maturation by organizing the spindle pole–specific PCM lattice.
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DOI：
10.1083/jcb.200908114
发表时间：
2010-01-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Delaval B;Doxsey SJ
通讯作者：
Doxsey SJ
影响因子：
4
作者：
Kim, Kyeongmi;Rhee, Kunsoo
通讯作者：
Rhee, Kunsoo
影响因子：
9.2
作者：
Conduit, Paul T.;Brunk, Kathrin;Raff, Jordan W.
通讯作者：
Raff, Jordan W.
影响因子：
3.3
作者：
Fong, Ka-Wing;Choi, Yuk-Kwan;Qi, Robert Z.
通讯作者：
Qi, Robert Z.
Cdk5rap2 与 Pericentrin 相互作用以维持发育中神经皮层的神经祖细胞库
影响因子：
16.2
作者：
Buchman, Joshua J.;Tseng, Huan-Chung;Tsai, Li-Huei
通讯作者：
Tsai, Li-Huei