Follicular fluid steroid and gonadotropic hormone levels and mitochondrial function from exosomes predict embryonic development.
Follicular fluid steroid and gonadotropic hormone levels and mitochondrial function from exosomes predict embryonic development.
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DOI：
10.3389/fendo.2022.1025523
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发表时间：
2022
影响因子：
5.2
通讯作者：
Guo, Wei
中科院分区：
文献类型：
--
作者：
Yu, Li;Liu, Miao;Xu, Shiji;Wang, Zhenxin;Liu, Te;Zhou, Jiaye;Zhang, Doudou;Dong, Xi;Pan, Baishen;Wang, Beili;Liu, Suying;Guo, Wei
人类卵泡液（FF）是一种复杂的生物液体，有助于卵母细胞发育的微环境。本研究的目的是评估体外受精周期中类固醇和促性腺激素水平以及线粒体功能在胚胎发育中的作用。这是一项对138名接受IVF/ICSI的女性进行的队列研究，包括来自109名不孕女性的136份FF样本。通过液相色谱-串联质谱法（LC-MS/MS）和免疫测定法检测FF类固醇和促性腺激素水平。qPCR检测FF exosomes线粒体电子传递链（ETC）复合物基因mRNA表达水平。对这些个体卵泡液浓度的分析表明，在卵母细胞发育成优质（TQ）囊胚的卵泡中LH和FSH浓度（LH：9.44 ± 2.32mIU/ml，FSH：9.32 ± 1.01mIU/ml）高于受精失败的卵泡（LH：5.30 ± 0.84mIU/ml，FSH：6.91 ± 0.62mIU/ml）。相比之下，产生TQ胚泡的卵母细胞的卵泡可的松浓度较低（12.20 ± 0.82mIU/ml）。受试者操作特征分析显示，FF、LH和FSH水平预测TQ囊胚的AUC值分别为0.711和0.747。线粒体ETC复合物I和III mRNA水平在TQ胚泡的FF外泌体中增加。相关分析显示，FF中ETC复合物I的mRNA水平与LH和FSH水平呈正相关。单个卵泡液中卵泡液类固醇激素和促性腺激素的水平在一定程度上可以预测胚胎的发育。此外，受损的外泌体线粒体功能障碍是导致胚胎发育中激素变化的潜在事件。
Human follicular fluid (FF) is a complex biological fluid that contributes to the micro-environment of oocyte development. The aim of this study was to evaluate the role of steroid and gonadotropic hormones levels and mitochondrial function in embryo development during in vitro fertilization cycles. This was a cohort study of 138 women receiving IVF/ICSI, including 136 FF samples from 109 infertile women. FF steroid and gonadotropic hormones levels were tested by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunoassays. The mRNA expression levels of mitochondrial electron transport chain (ETC) complex genes from FF exosomes were detected by qPCR. Analysis of these individual FF concentrations revealed that LH and FSH concentrations were higher in follicles in which the oocyte developed into a top quality (TQ) blastocyst (LH: 9.44 ± 2.32mIU/ml, FSH: 9.32 ± 1.01mIU/ml) than those in which there was a failure of fertilization (LH: 5.30 ± 0.84mIU/ml, FSH: 6.91 ± 0.62mIU/ml). In contrast, follicular cortisone concentrations were lower for oocytes that resulted in a TQ blastocyst (12.20 ± 0.82mIU/ml). The receiver operating characteristic analysis showed that FF LH and FSH levels predicted TQ blastocyst with excellent AUC value of 0.711 and 0.747. Mitochondrial ETC complex I and III mRNA levels were increased in the FF exosomes of TQ blastocyst. Correlation analysis showed that mRNA levels of ETC complex I was positively correlated with LH and FSH levels in FF. The levels of FF steroid and gonadotropic hormones from single follicle can predetermine subsequent embryo development to some extent. Furthermore, impaired exosome mitochondrial dysfunction is a potiential event that causes hormone change in embryo development.
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