Differential gene expression in nasal airway epithelium from overweight or obese youth with asthma.
Differential gene expression in nasal airway epithelium from overweight or obese youth with asthma.
复制标题
超重或肥胖青少年哮喘患者鼻气道上皮的差异基因表达。
DOI：
10.1111/pai.13776
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发表时间：
2022-04
影响因子：
4.4
通讯作者：
Celedon, Juan C.
中科院分区：
文献类型：
--
作者：
Xu, Zhongli;Forno, Erick;Acosta-Perez, Edna;Han, Yueh-Ying;Rosser, Franziska;Manni, Michelle L.;Canino, Glorisa;Chen, Wei;Celedon, Juan C.
The mechanisms underlying the known link between overweight/obesity and childhood asthma are unclear. We aimed to identify differentially expressed genes and pathways associated with obesity-related asthma through a transcriptomic analysis of nasal airway epithelium. We compared the whole transcriptome in nasal airway epithelium of youth with overweight or obesity and asthma with that of youth of normal weight and asthma, using RNA sequencing data from a cohort of 235 Puerto Ricans aged 9 to 20 years (EVA-PR) and an independent cohort of 66 children aged 6 to 16 years in Pittsburgh (VDKA). Differential expression analysis adjusting for age, sex, sequencing plate number, sample sorting protocol, and the first five principal components was performed independently in each cohort. Results from the two cohorts were combined in a transcriptome-wide meta-analysis. Gene enrichment and network analyses were performed on top genes. In the meta-analysis, 29 genes were associated with obesity-related asthma at an FDR-adjusted P <0.05, including pro-inflammatory genes known to be differentially expressed in adipose tissue of obese subjects (e.g., CXCL11, CXCL10, and CXCL9) and several novel genes. Functional enrichment analyses showed that pathways for interferon signaling, and innate and adaptive immune responses were down-regulated in overweight/obese youth with asthma, while pathways related to ciliary structure or function were up-regulated. Upstream regulatory analysis predicted significant inhibition of the IRF7 pathway. Network analyses identified “hub” genes like GBP5 and SOCS1. Our transcriptome-wide analysis of nasal airway epithelium identified biologically plausible genes and pathways for obesity-related asthma in youth.
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影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
120.7
作者：
Forno, Erick;Bacharier, Leonard B.;Celedon, Juan C.
通讯作者：
Celedon, Juan C.
DOI：
10.1126/science.aaa1578
发表时间：
2015-04-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ciancanelli MJ;Huang SX;Luthra P;Garner H;Itan Y;Volpi S;Lafaille FG;Trouillet C;Schmolke M;Albrecht RA;Israelsson E;Lim HK;Casadio M;Hermesh T;Lorenzo L;Leung LW;Pedergnana V;Boisson B;Okada S;Picard C;Ringuier B;Troussier F;Chaussabel D;Abel L;Pellier I;Notarangelo LD;García-Sastre A;Basler CF;Geissmann F;Zhang SY;Snoeck HW;Casanova JL
通讯作者：
Casanova JL
影响因子：
14.2
作者：
Forno, Erick;Acosta-Perez, Edna;Celedon, Juan C.
通讯作者：
Celedon, Juan C.
影响因子：
24.5
作者：
Baselli, Guido Alessandro;Dongiovanni, Paola;Valenti, Luca
通讯作者：
Valenti, Luca