Red Cabbage Juice-Mediated Gut Microbiota Modulation Improves Intestinal Epithelial Homeostasis and Ameliorates Colitis.
Red Cabbage Juice-Mediated Gut Microbiota Modulation Improves Intestinal Epithelial Homeostasis and Ameliorates Colitis.
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红甘蓝汁介导的肠道微生物群调节可改善肠上皮稳态并减轻结肠炎。
DOI：
10.3390/ijms25010539
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发表时间：
2023-12-30
影响因子：
5.6
通讯作者：
Rachagani, Satyanarayana
中科院分区：
文献类型：
--
作者：
Wilson, Emily Jean;Natesh, Nagabhishek Sirpu;Ghadermazi, Parsa;Pothuraju, Ramesh;Prajapati, Dipakkumar R.;Pandey, Sanjit;Kaifi, Jussuf T.;Dodam, John R.;Bryan, Jeffrey N.;Lorson, Christian L.;Watrelot, Aude A.;Foster, Jason M.;Mansell, Thomas J.;Chan, Siu Hung Joshua;Batra, Surinder K.;Subbiah, Jeyamkondan;Rachagani, Satyanarayana
关键词：
肠道微生物群在炎症性肠病（IBD）中起着至关重要的作用，并且可以通过微生物衍生的代谢物潜在地预防IBD，使其成为一种有前途的治疗途径。最近的证据表明，尽管潜在的机制尚不清楚，但红甘蓝汁（RCJ）可抑制右旋糖酐硫酸钠（DSS）诱导的小鼠结肠炎。因此，该研究旨在揭示RCJ调节肠道微生物群以减轻DSS诱导的小鼠结肠炎的分子机制。使用C57BL/6J小鼠，我们通过两个周期的3%DSS来评估RCJ在DSS诱导的结肠炎中的保护作用。每天用PBS或RCJ管饲小鼠直到终点，并通过鸟枪宏基因组学分析肠道微生物群组成。RCJ治疗显著改善了小鼠的体重（p ≤ 0.001）、存活率（p <0.001）并降低了疾病活动指数（DAI）评分。此外，与DSS组相比，RCJ通过增强RCJ + DSS治疗小鼠中保护性结肠粘蛋白（p <0.001）和紧密连接蛋白（p ≤ 0.01）的表达来改善结肠屏障完整性。鸟枪式宏基因组分析显示，产生短链脂肪酸（SCFAs）的细菌富集（p <0.05），导致过氧化物酶体激活受体γ（PPAR-γ）激活增加（p ≤ 0.001）。这反过来又导致核因子κ B（NF κ B）信号通路的抑制，导致炎性细胞因子和趋化因子的产生减少。我们的研究证明了DSS诱导的小鼠模型中的结肠炎缓解，展示了RCJ作为肠道微生物群和代谢物的潜在调节剂，对IBD的预防和治疗具有重要意义。
Gut microbiota plays a crucial role in inflammatory bowel diseases (IBD) and can potentially prevent IBD through microbial-derived metabolites, making it a promising therapeutic avenue. Recent evidence suggests that despite an unclear underlying mechanism, red cabbage juice (RCJ) alleviates Dextran Sodium Sulfate (DSS)-induced colitis in mice. Thus, the study aims to unravel the molecular mechanism by which RCJ modulates the gut microbiota to alleviate DSS-induced colitis in mice. Using C57BL/6J mice, we evaluated RCJ’s protective role in DSS-induced colitis through two cycles of 3% DSS. Mice were daily gavaged with PBS or RCJ until the endpoint, and gut microbiota composition was analyzed via shotgun metagenomics. RCJ treatment significantly improved body weight (p ≤ 0.001), survival in mice (p < 0.001) and reduced disease activity index (DAI) scores. Further, RCJ improved colonic barrier integrity by enhancing the expression of protective colonic mucins (p < 0.001) and tight junction proteins (p ≤ 0.01) in RCJ + DSS-treated mice compared to the DSS group. Shotgun metagenomic analysis revealed an enrichment of short-chain fatty acids (SCFAs)-producing bacteria (p < 0.05), leading to increased Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ) activation (p ≤ 0.001). This, in turn, resulted in repression of the nuclear factor κB (NFκB) signaling pathway, causing decreased production of inflammatory cytokines and chemokines. Our study demonstrates colitis remission in a DSS-induced mouse model, showcasing RCJ as a potential modulator for gut microbiota and metabolites, with promising implications for IBD prevention and treatment.
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影响因子：
11.1
作者：
Deleu S;Machiels K;Raes J;Verbeke K;Vermeire S
通讯作者：
Vermeire S
DOI：
10.1126/science.aam9949
发表时间：
2017-08-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Byndloss MX;Olsan EE;Rivera-Chávez F;Tiffany CR;Cevallos SA;Lokken KL;Torres TP;Byndloss AJ;Faber F;Gao Y;Litvak Y;Lopez CA;Xu G;Napoli E;Giulivi C;Tsolis RM;Revzin A;Lebrilla CB;Bäumler AJ
通讯作者：
Bäumler AJ
影响因子：
7.7
作者：
Beghini F;McIver LJ;Blanco-Míguez A;Dubois L;Asnicar F;Maharjan S;Mailyan A;Manghi P;Scholz M;Thomas AM;Valles-Colomer M;Weingart G;Zhang Y;Zolfo M;Huttenhower C;Franzosa EA;Segata N
通讯作者：
Segata N
DOI：
10.3390/molecules27020542
发表时间：
2022-01-15
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Cheng Y;Savits JR;Watrelot AA
通讯作者：
Watrelot AA
影响因子：
3.4
作者：
Arda-Pirincci, Pelin;Aykol-Celik, Guliz
通讯作者：
Aykol-Celik, Guliz