Intravital Imaging Reveals Ghost Fibers as Architectural Units Guiding Myogenic Progenitors during Regeneration.
Intravital Imaging Reveals Ghost Fibers as Architectural Units Guiding Myogenic Progenitors during Regeneration.
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活体成像揭示幽灵纤维作为在再生过程中指导肌源性祖细胞的结构单元。
DOI：
10.1016/j.stem.2015.11.005
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发表时间：
2016
期刊：
影响因子：
23.9
通讯作者：
Fan,Chen-Ming
中科院分区：
文献类型：
--
作者：
Webster,MicahT;Manor,Uri;Lippincott-Schwartz,Jennifer;Fan,Chen-Ming
常驻干细胞和它们的直接祖细胞如何重建损伤前组织和大小的组织，以实现成比例的再生，目前还没有很好的理解。使用3D，延时活体成像直接可视化的肌肉再生过程中活的小鼠，我们报告说，细胞外基质残余物从受伤的骨骼肌纤维，“鬼纤维”，肌肉干/祖细胞的行为在成比例再生。干细胞在无损伤时静止不动，而其活化的祖细胞在损伤后迁移和分裂。出乎意料的是，分裂和迁移主要是沿血影纤维纵轴沿着双向取向的，允许祖细胞在血影纤维中扩散。重定向鬼纤维影响肌原性祖细胞的迁移路径和分裂平面，导致再生肌纤维的紊乱。我们的结论是，幽灵纤维是组织损伤后成比例再生所必需的自主结构单位。这一发现加强了制造模拟活组织基质用于组织再生治疗的生物工程基质的需要。
How resident stem cells and their immediate progenitors rebuild tissues of pre-injury organization and size for proportional regeneration is not well understood. Using 3D, time-lapse intravital imaging for direct visualization of the muscle regeneration process in live mice, we report that extracellular matrix remnants from injured skeletal muscle fibers, "ghost fibers," govern muscle stem/progenitor cell behaviors during proportional regeneration. Stem cells were immobile and quiescent without injury whereas their activated progenitors migrated and divided after injury. Unexpectedly, divisions and migration were primarily bi-directionally oriented along the ghost fiber longitudinal axis, allowing for spreading of progenitors throughout ghost fibers. Re-orienting ghost fibers impacted myogenic progenitors' migratory paths and division planes, causing disorganization of regenerated muscle fibers. We conclude that ghost fibers are autonomous, architectural units necessary for proportional regeneration after tissue injury. This finding reinforces the need to fabricate bioengineered matrices that mimic living tissue matrices for tissue regeneration therapy.
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巩膜炎的诊断和治疗。
DOI：
--
发表时间：
1980
期刊：
Ophthalmology (Rochester, Minn.)
影响因子：
--
作者：
P. Watson
通讯作者：
P. Watson
抗炎药物在角叉菜胶引起的大鼠足部水肿中的相互作用。
影响因子：
3.5
作者：
K. Swingle;T. J. Grant;L. W. Jaques;D. Kvam
通讯作者：
D. Kvam
DOI：
--
发表时间：
1979
期刊：
影响因子：
--
作者：
P. Dodge;D. A. Brodie;B. Mitchell
通讯作者：
B. Mitchell
治疗关节炎的新药。
影响因子：
4.2
作者：
Charley J. Smyth
通讯作者：
Charley J. Smyth