Identification of an O-antigen chain length regulator, WzzP, in Porphyromonas gingivalis.
Identification of an O-antigen chain length regulator, WzzP, in Porphyromonas gingivalis.
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DOI：
10.1002/mbo3.84
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发表时间：
2013-06
期刊：
影响因子：
3.4
通讯作者：
Nakayama, Koji
中科院分区：
文献类型：
--
作者：
Shoji, Mikio;Yukitake, Hideharu;Sato, Keiko;Shibata, Yasuko;Naito, Mariko;Aduse-Opoku, Joseph;Abiko, Yoshimitsu;Curtis, Michael A.;Nakayama, Koji
牙周病原菌牙龈卟啉单胞菌具有两种不同的脂多糖（LPS），分别命名为 O-LPS 和 A-LPS，它们分别是传统的 O 抗原多糖和阴离子多糖，两者均与脂质 A 核心相连。然而，LPS生物合成的精确机制仍有待确定。在本研究中，我们通过转座子诱变分离出无色素突变体，并鉴定转座子被插入到编码序列PGN_2005中，该序列编码牙龈卟啉单胞菌ATCC 33277的假设蛋白。我们发现（i）从PGN_2005突变体中纯化的LPS比野生型的LPS更短； (ii) PGN_2005蛋白位于内膜部分； (iii)PGN_2005基因赋予大肠杆菌wzz突变体Wzz活性。这些结果表明，被命名为WzzP的PGN_2005蛋白是牙龈卟啉单胞菌中Wzz蛋白的功能同源物。 WzzP 与常规 Wzz 蛋白的氨基酸序列比较表明，WzzP 在 C 端区域有一个额外的片段。此外，我们确定PGN_1896和PGN_1233蛋白以及PGN_1033蛋白似乎分别是参与LPS生物合成的WbaP同源蛋白和Wzx同源蛋白。
The periodontal pathogen Porphyromonas gingivalis has two different lipopolysaccharides (LPSs) designated O-LPS and A-LPS, which are a conventional O-antigen polysaccharide and an anionic polysaccharide that are both linked to lipid A-cores, respectively. However, the precise mechanisms of LPS biosynthesis remain to be determined. In this study, we isolated a pigment-less mutant by transposon mutagenesis and identified that the transposon was inserted into the coding sequence PGN_2005, which encodes a hypothetical protein of P. gingivalis ATCC 33277. We found that (i) LPSs purified from the PGN_2005 mutant were shorter than those of the wild type; (ii) the PGN_2005 protein was located in the inner membrane fraction; and (iii) the PGN_2005 gene conferred Wzz activity upon an Escherichia coli wzz mutant. These results indicate that the PGN_2005 protein, which was designated WzzP, is a functional homolog of the Wzz protein in P. gingivalis. Comparison of amino acid sequences among WzzP and conventional Wzz proteins indicated that WzzP had an additional fragment at the C-terminal region. In addition, we determined that the PGN_1896 and PGN_1233 proteins and the PGN_1033 protein appear to be WbaP homolog proteins and a Wzx homolog protein involved in LPS biosynthesis, respectively.
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DOI：
10.1126/science.1227215
发表时间：
2012-09-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chng SS;Xue M;Garner RA;Kadokura H;Boyd D;Beckwith J;Kahne D
通讯作者：
Kahne D
影响因子：
3.1
作者：
Kondo, Yoshio;Ohara, Naoya;Nakayama, Koji
通讯作者：
Nakayama, Koji
影响因子：
3.6
作者：
BASTIN, DA;STEVENSON, G;REEVES, PR
通讯作者：
REEVES, PR
影响因子：
3.2
作者：
Kintz, Erica;Scarff, Jennifer M.;Goldberg, Joanna B.
通讯作者：
Goldberg, Joanna B.
DOI：
10.1073/pnas.0500044102
发表时间：
2005-02-22
影响因子：
11.1
作者：
Feldman, MF;Wacker, M;Aebi, M
通讯作者：
Aebi, M