Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways.
Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways.
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来自HIV暴露的个体的口服和肛门微生物组：与宿主相关因素在分类单元组成和代谢途径中的作用。
DOI：
10.1038/s41522-023-00413-4
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发表时间：
2023-07-12
影响因子：
9.2
通讯作者：
Abba, Martin C.
中科院分区：
文献类型：
--
作者：
Lacunza, Ezequiel;Fink, Valeria;Salas, Maria E.;Canzoneri, Romina;Naipauer, Julian;Williams, Sion;Coso, Omar;Sued, Omar;Cahn, Pedro;Mesri, Enrique A.;Abba, Martin C.
有证据表明，微生物群在HIV免疫发病机制和相关并发症中发挥着重要作用。这项研究的目的是描述有性行为的男性(MSM)和变性人女性(TGW)的口腔和肛门微生物群的特征，有无HIV感染。来自78名参与者的130份口腔和肛门脱氧核糖核酸样本进行了鸟枪式元基因组测序，以进行进一步的微生物组分析。在与HIV感染、性别、性行为、CD4+T细胞计数、抗逆转录病毒治疗(ART)以及是否存在HPV相关的癌前病变相关的受试者中，微生物组组成存在显著差异。结果证实了在这一艾滋病毒高危人群中发生了致癌病毒。HIV相关病例的口腔微生物群显示出与牙周病发病机制相关的细菌的丰富。相反，在感染HIV的受试者中，肛门细菌显示出显著的减少(Coproins Comes，Finegoldia Magna，Blautia obum，Catenibacmitsuokai)。TGW显示与性传播有关的物种丰富，这表明大多数被招募的TGW是或曾经是性工作者。在HIV感染者中，普氏杆菌和梭状杆菌与肛门癌前病变呈正相关。霍尔德曼氏菌和C.Comes的丰富与可检测到的病毒载量和未经ART治疗的患者有关。代谢途径明显受到与性行为或HIV致病相关的主要因素的影响。基因家族分析确定细菌基因特征是艾滋病毒/艾滋病相关恶性肿瘤的潜在预后和预测生物标记物。结论：可接触部位的微生物特征是预测癌前病变的潜在生物标志物，也是HIV免疫发病的治疗靶点。
Evidence indicates that the microbiome plays a significant role in HIV immunopathogenesis and associated complications. This study aimed to characterize the oral and anal microbiome of Men who have Sex with Men (MSM) and Transgender Women (TGW), with and without HIV. One hundred and thirty oral and anal DNA-derived samples were obtained from 78 participants and subjected to shotgun metagenomics sequencing for further microbiome analysis. Significant differences in the microbiome composition were found among subjects associated with HIV infection, gender, sex behavior, CD4+ T-cell counts, antiretroviral therapy (ART), and the presence of HPV-associated precancerous anal lesions. Results confirm the occurrence of oncogenic viromes in this high HIV-risk population. The oral microbiome in HIV-associated cases exhibited an enrichment of bacteria associated with periodontal disease pathogenesis. Conversely, anal bacteria showed a significant decrease in HIV-infected subjects (Coprococcus comes, Finegoldia magna, Blautia obeum, Catenibacterium mitsuokai). TGW showed enrichment in species related to sexual transmission, which concurs that most recruited TGW are or have been sex workers. Prevotella bivia and Fusobacterium gonidiaformans were positively associated with anal precancerous lesions among HIV-infected subjects. The enrichment of Holdemanella biformis and C. comes was associated with detectable viral load and ART-untreated patients. Metabolic pathways were distinctly affected by predominant factors linked to sexual behavior or HIV pathogenesis. Gene family analysis identified bacterial gene signatures as potential prognostic and predictive biomarkers for HIV/AIDS-associated malignancies. Conclusions: Identified microbial features at accessible sites are potential biomarkers for predicting precancerous anal lesions and therapeutic targets for HIV immunopathogenesis.
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影响因子：
64.8
作者：
通讯作者：
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影响因子：
14.9
作者：
Caspi R;Billington R;Ferrer L;Foerster H;Fulcher CA;Keseler IM;Kothari A;Krummenacker M;Latendresse M;Mueller LA;Ong Q;Paley S;Subhraveti P;Weaver DS;Karp PD
通讯作者：
Karp PD
影响因子：
7.7
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Beghini F;McIver LJ;Blanco-Míguez A;Dubois L;Asnicar F;Maharjan S;Mailyan A;Manghi P;Scholz M;Thomas AM;Valles-Colomer M;Weingart G;Zhang Y;Zolfo M;Huttenhower C;Franzosa EA;Segata N
通讯作者：
Segata N
影响因子：
2.3
作者：
Austin MN;Rabe LK;Srinivasan S;Fredricks DN;Wiesenfeld HC;Hillier SL
通讯作者：
Hillier SL
影响因子：
3.2
作者：
Castellsague, Xavier;Ault, Kevin A.;Velicer, Christine
通讯作者：
Velicer, Christine