A multi-signal fluorescent probe for simultaneously distinguishing and sequentially sensing cysteine/homocysteine, glutathione, and hydrogen sulfide in living cells.
A multi-signal fluorescent probe for simultaneously distinguishing and sequentially sensing cysteine/homocysteine, glutathione, and hydrogen sulfide in living cells.
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一种多信号荧光探针，用于同时区分和顺序检测活细胞中的半胱氨酸/高半胱氨酸、谷胱甘肽和硫化氢
DOI：
10.1039/c7sc00423k
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发表时间：
2017-09-01
期刊：
影响因子：
8.4
通讯作者：
Lin W
中科院分区：
文献类型：
--
作者：
He L;Yang X;Xu K;Kong X;Lin W
设计了一种多信号荧光探针，用于同时区分和顺序检测活细胞中的半胱氨酸/同型半胱氨酸、谷胱甘肽和硫化氢。生物硫醇具有紧密的生成和代谢途径网络，是细胞中必需的活性硫物种（RSS），在人体生理学中发挥着重要作用。然而，生物硫醇具有高度相似的化学结构和性质，导致同时区分它们是一个巨大的挑战。在此，我们开发了一种独特的荧光探针（HMN），不仅可以同时区分Cys/Hcy、GSH和H2S，而且首次使用多通道荧光模式顺序检测Cys/Hcy/GSH和H2S。当响应各自的生物硫醇时，强大的探针在三个不同的发射带（蓝-绿-红）处表现出多组荧光信号。新探针还可以通过蓝色和红色发射带荧光的变化来检测不同浓度水平的H2S。此外，新型探针HMN能够通过三色荧光成像区分并顺序感知生物环境中的生物硫醇。我们期望鲁棒探针HMN的开发将为设计用于生物硫醇的区分和顺序检测的荧光探针提供强大的策略，并为探索生物硫醇在各种生理和病理条件下的相互关联作用提供有前途的工具。
A multi-signal fluorescent probe was engineered for simultaneously distinguishing and sequentially sensing cysteine/homocysteine, glutathione, and hydrogen sulfide in living cells. Biothiols, which have a close network of generation and metabolic pathways among them, are essential reactive sulfur species (RSS) in the cells and play vital roles in human physiology. However, biothiols possess highly similar chemical structures and properties, resulting in it being an enormous challenge to simultaneously discriminate them from each other. Herein, we develop a unique fluorescent probe (HMN) for not only simultaneously distinguishing Cys/Hcy, GSH, and H2S from each other, but also sequentially sensing Cys/Hcy/GSH and H2S using a multi-channel fluorescence mode for the first time. When responding to the respective biothiols, the robust probe exhibits multiple sets of fluorescence signals at three distinct emission bands (blue-green-red). The new probe can also sense H2S at different concentration levels with changes of fluorescence at the blue and red emission bands. In addition, the novel probe HMN is able to discriminate and sequentially sense biothiols in biological environments via three-color fluorescence imaging. We expect that the development of the robust probe HMN will provide a powerful strategy to design fluorescent probes for the discrimination and sequential detection of biothiols, and offer a promising tool for exploring the interrelated roles of biothiols in various physiological and pathological conditions.
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