Distal C terminus of CaV1.2 channels plays a crucial role in the neural differentiation of dental pulp stem cells.
Distal C terminus of CaV1.2 channels plays a crucial role in the neural differentiation of dental pulp stem cells.
复制标题
CaV1.2 通道的远端 C 末端在牙髓干细胞的神经分化中起着至关重要的作用。
DOI：
10.1371/journal.pone.0081332
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhao S
中科院分区：
文献类型：
--
作者：
Ge J;Ju Y;Xue Z;Feng Y;Huang X;Liu H;Zhao S
L型电压依赖性CaV1.2通道在维持细胞内钙稳态中起重要作用，并影响多种细胞过程。据报道，CaV1.2通道的C末端切割存在于多种类型的可兴奋细胞中，但其在非可兴奋细胞中的表达和可能的作用仍不清楚。本研究的目的是确定CaV1.2通道的远端C端片段是否存在于大鼠牙髓干细胞及其在大鼠牙髓干细胞神经分化中的可能作用。我们通过短发夹RNA（shRNA）产生稳定的CaV1.2敲低细胞。缺失CaV1.2通道远端C端的大鼠牙髓干细胞丧失向神经细胞分化的潜能。CaV1.2远端C端的重新表达挽救了敲低内源性CaV1.2对大鼠牙髓干细胞神经分化的影响，表明CaV1.2远端C端是大鼠牙髓干细胞神经分化所必需的。这些结果提供了新的见解电压门控钙通道在干细胞分化过程中的作用。
L-type voltage-dependent CaV1.2 channels play an important role in the maintenance of intracellular calcium homeostasis, and influence multiple cellular processes. C-terminal cleavage of CaV1.2 channels was reported in several types of excitable cells, but its expression and possible roles in non-excitable cells is still not clear. The aim of this study was to determine whether distal C-terminal fragment of CaV1.2 channels is present in rat dental pulp stem cells and its possible role in the neural differentiation of rat dental pulp stem cells. We generated stable CaV1.2 knockdown cells via short hairpin RNA (shRNA). Rat dental pulp stem cells with deleted distal C-terminal of CaV1.2 channels lost the potential of differentiation to neural cells. Re-expression of distal C-terminal of CaV1.2 rescued the effect of knocking down the endogenous CaV1.2 on the neural differentiation of rat dental pulp stem cells, indicating that the distal C-terminal of CaV1.2 is required for neural differentiation of rat dental pulp stem cells. These results provide new insights into the role of voltage-gated Ca2+ channels in stem cells during differentiation.
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影响因子：
20.1
作者：
Schroder E;Byse M;Satin J
通讯作者：
Satin J
DOI：
10.1016/j.bbrc.2009.04.150
发表时间：
2009-07-10
影响因子：
3.1
作者：
Karnabi, Eddy;Qu, Yongxia;Mancarella, Salvatore;Yue, Yuankun;Wadgaonkar, Raj;Boutjdir, Mohamed
通讯作者：
Boutjdir, Mohamed
影响因子：
3.7
作者：
Janebodin K;Horst OV;Ieronimakis N;Balasundaram G;Reesukumal K;Pratumvinit B;Reyes M
通讯作者：
Reyes M
DOI：
10.1073/pnas.88.23.10778
发表时间：
1991-12-01
影响因子：
11.1
作者：
DEJONGH, KS;WARNER, C;CATTERALL, WA
通讯作者：
CATTERALL, WA
影响因子：
2.5
作者：
Guo, Zixuan;Shi, Fang;Wang, Xiaomin
通讯作者：
Wang, Xiaomin