Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice.
Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice.
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DOI：
10.1371/journal.pone.0027526
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Reyes M
中科院分区：
文献类型：
--
作者：
Janebodin K;Horst OV;Ieronimakis N;Balasundaram G;Reesukumal K;Pratumvinit B;Reyes M
牙髓干细胞（DPSC）被证明存在于牙齿内，并在牙本质再生中发挥重要作用。DPSC首先从人类牙齿中分离和表征，大多数研究都集中在将这种成体干细胞用于临床应用。然而，小鼠DPSC尚未得到很好的表征，其来源尚未阐明。在此，我们检查了鼠DPSC是否是神经嵴衍生的，并确定了它们的体外和体内能力。新生鼠牙髓DPSCs表达胚胎干细胞和神经嵴相关基因，但不表达中胚层相关基因。从Wnt 1-Cre/R26 R-LacZ模型（神经嵴来源组织的报告基因）分离的细胞表明DPSC是Wnt 1标记的，因此是神经嵴来源的。DPSCs在神经嵴谱系中表现为成牙本质细胞、软骨细胞、脂肪细胞、神经元和平滑肌的多向分化。经羟基磷灰石/磷酸三钙体内皮下移植后，根据组织/细胞形态学和特异性抗体染色，克隆分化为成牙本质样细胞并产生牙本质样结构。相反，骨髓基质细胞（BMSCs）产生成骨样细胞，并产生骨样结构。有趣的是，DPSC移植物中的毛细血管分布显示出与成牙本质细胞非常接近，而在BMSC移植物中，骨凝聚远离毛细血管，类似于前者中的牙本质生成与后者中的骨生成。因此，我们证明了存在的神经嵴衍生的DPSC分化能力，成颅间充质组织和其他神经嵴衍生的组织。反过来，DPSC有望成为再生颅间充质和其他神经嵴衍生组织的来源。
Dental pulp stem cells (DPSCs) are shown to reside within the tooth and play an important role in dentin regeneration. DPSCs were first isolated and characterized from human teeth and most studies have focused on using this adult stem cell for clinical applications. However, mouse DPSCs have not been well characterized and their origin(s) have not yet been elucidated. Herein we examined if murine DPSCs are neural crest derived and determined their in vitro and in vivo capacity. DPSCs from neonatal murine tooth pulp expressed embryonic stem cell and neural crest related genes, but lacked expression of mesodermal genes. Cells isolated from the Wnt1-Cre/R26R-LacZ model, a reporter of neural crest-derived tissues, indicated that DPSCs were Wnt1-marked and therefore of neural crest origin. Clonal DPSCs showed multi-differentiation in neural crest lineage for odontoblasts, chondrocytes, adipocytes, neurons, and smooth muscles. Following in vivo subcutaneous transplantation with hydroxyapatite/tricalcium phosphate, based on tissue/cell morphology and specific antibody staining, the clones differentiated into odontoblast-like cells and produced dentin-like structure. Conversely, bone marrow stromal cells (BMSCs) gave rise to osteoblast-like cells and generated bone-like structure. Interestingly, the capillary distribution in the DPSC transplants showed close proximity to odontoblasts whereas in the BMSC transplants bone condensations were distant to capillaries resembling dentinogenesis in the former vs. osteogenesis in the latter. Thus we demonstrate the existence of neural crest-derived DPSCs with differentiation capacity into cranial mesenchymal tissues and other neural crest-derived tissues. In turn, DPSCs hold promise as a source for regenerating cranial mesenchyme and other neural crest derived tissues.
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DOI：
10.1161/atvbaha.109.191965
发表时间：
2009-10
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Grabski AD;Shimizu T;Deou J;Mahoney WM Jr;Reidy MA;Daum G
通讯作者：
Daum G
DOI：
10.1073/pnas.0501263102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Amoh, Y;Li, L;Hoffman, RM
通讯作者：
Hoffman, RM
影响因子：
7.6
作者：
Feng, JQ;Huang, H;Mishina, Y
通讯作者：
Mishina, Y
影响因子：
20.3
作者：
GRONTHOS, S;GRAVES, SE;SIMMONS, PJ
通讯作者：
SIMMONS, PJ
影响因子：
4.2
作者：
Huang, George T-J.;Shagramanova, Kristina;Chan, Selina W.
通讯作者：
Chan, Selina W.