Distinct contribution of cone photoreceptor subtypes to the mammalian biological clock.
Distinct contribution of cone photoreceptor subtypes to the mammalian biological clock.
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锥形感受器亚型对哺乳动物生物时钟的不同贡献。
DOI：
10.1073/pnas.2024500118
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发表时间：
2021-06-01
影响因子：
11.1
通讯作者：
Meijer JH
中科院分区：
文献类型：
--
作者：
van Diepen HC;Schoonderwoerd RA;Ramkisoensing A;Janse JAM;Hattar S;Meijer JH
我们的生物钟与环境昼夜周期的同步关键取决于每天的光照。在这里，我们表明，锥传递不同的光信息的时钟进行记录的时钟神经元在自由移动的小鼠与锥作为他们唯一的光感受器。这与期望黑视素和视杆细胞专门发挥这一作用相反。此外，我们表明，特别是短波长敏感锥相比，中波长敏感锥是重要的。锥细胞作用的证据表明，生物钟对广谱的颜色而不是蓝光做出反应，这可以用来加强人类的生物钟。环境光检测对于生物钟与外部太阳周期的同步是重要的。光信号被发送到视交叉上核（SCN），这是主要昼夜节律起搏点的所在地。已经假设视锥光感受器对同步化的贡献最小。然而，在这里，我们发现，锥光感受器是足够的介导夹带和传输光信息的SCN，在只有锥作为功能性光感受器的小鼠进行评估。使用在体电生理记录在SCN的自由移动的锥只有小鼠，我们观察到SCN神经元活动的光响应60秒脉冲的紫外线（UV）（λ max 365 nm）和绿色（λ max 505 nm）光。较高的紫外光辐照度导致SCN神经元活动的辐照度依赖性增强，而较高的绿色光辐照度导致持续反应减少，仅剩下短暂反应。SCN神经元活动的响应衰减的半最大时间为10.9分钟的UV光和不到一分钟的绿色光，表明短波长敏感和中波长敏感的视锥细胞的SCN响应之间的差异输入。此外，我们表明，紫外光是更有效的光夹带比绿色光。基于在仅视锥细胞的小鼠中缺乏完全持续的反应，我们证实了快速交替的光水平，而不是缓慢交替的光，引起了实质性的相移。总之，我们的数据提供了强有力的证据，锥类型有助于光夹带和差异影响的SCN的电活动水平。
Synchronization of our biological clocks to the environmental day–night cycle critically depends on daily exposure to light. Here, we show that cones transmit distinct photic information to the clock by performing recordings of clock neurons in freely moving mice with cones as their only photoreceptors. This is in contrast to the expectation that exclusively melanopsin and rods fulfil this role. Moreover, we show that especially short-wavelength–sensitive cones as compared to mid-wavelength–sensitive cones are important. The evidence for a role for cones implicates that clocks respond to a broad spectrum of colors rather than to blue light, which can be used to strengthen the clock in humans. Ambient light detection is important for the synchronization of the circadian clock to the external solar cycle. Light signals are sent to the suprachiasmatic nuclei (SCN), the site of the major circadian pacemaker. It has been assumed that cone photoreceptors contribute minimally to synchronization. Here, however, we find that cone photoreceptors are sufficient for mediating entrainment and transmitting photic information to the SCN, as evaluated in mice that have only cones as functional photoreceptors. Using in vivo electrophysiological recordings in the SCN of freely moving cone-only mice, we observed light responses in SCN neuronal activity in response to 60-s pulses of both ultraviolet (UV) (λmax 365 nm) and green (λmax 505 nm) light. Higher irradiances of UV light led to irradiance-dependent enhancements in SCN neuronal activity, whereas higher irradiances of green light led to a reduction in the sustained response with only the transient response remaining. Responses in SCN neuronal activity decayed with a half-max time of ∼9 min for UV light and less than a minute for green light, indicating differential input between short-wavelength–sensitive and mid-wavelength–sensitive cones for the SCN responsiveness. Furthermore, we show that UV light is more effective for photoentrainment than green light. Based on the lack of a full sustained response in cone-only mice, we confirmed that rapidly alternating light levels, rather than slowly alternating light, caused substantial phase shifts. Together, our data provide strong evidence that cone types contribute to photoentrainment and differentially affect the electrical activity levels of the SCN.
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影响因子：
25
作者：
Altimus, Cara M.;Gueler, Ali D.;Alam, Nazia M.;Arman, A. Cyrus;Prusky, Glen T.;Sampath, Alapakkam P.;Hattar, Samer
通讯作者：
Hattar, Samer
影响因子：
2.9
作者：
MEIJER, JH;RUSAK, B;GANSHIRT, G
通讯作者：
GANSHIRT, G
影响因子：
16.2
作者：
Lall, Gurprit S.;Revell, Victoria L.;Momiji, Hiroshi;Al Enezi, Jazi;Altimus, Cara M.;Gueler, Ali D.;Aguilar, Carlos;Cameron, Morven A.;Allender, Susan;Hankins, Mark W.;Lucas, Robert J.
通讯作者：
Lucas, Robert J.
DOI：
10.1073/pnas.250478897
发表时间：
2000-12-05
影响因子：
11.1
作者：
Calvert, PD;Krasnoperova, NV;Lem, J
通讯作者：
Lem, J
DOI：
10.1523/jneurosci.3656-10.2010
发表时间：
2010-12-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Schmidt TM;Kofuji P
通讯作者：
Kofuji P