Fatty acid composition of the follicular fluid of normal weight, overweight and obese women undergoing assisted reproductive treatment: a descriptive cross-sectional study.
Fatty acid composition of the follicular fluid of normal weight, overweight and obese women undergoing assisted reproductive treatment: a descriptive cross-sectional study.
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DOI：
10.1186/1477-7827-12-13
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发表时间：
2014-02-05
期刊：
影响因子：
--
通讯作者：
Leroy JL
中科院分区：
文献类型：
--
作者：
Valckx SD;Arias-Alvarez M;De Pauw I;Fievez V;Vlaeminck B;Fransen E;Bols PE;Leroy JL
已有文献证明，成熟的卵母细胞非常容易受到其微环境-卵泡液(FF)变化的影响。最近的研究集中在这种胎儿中的不同成分，如激素、生长因子和代谢物，以及它们的浓度如何因饮食和母亲的新陈代谢健康而改变。脂肪酸(FA)是影响卵母细胞成熟和随后胚胎发育的潜在因素。然而，目前还缺乏对每种脂类成分的特定的FFFA组成以及BMI如何影响这一点的彻底研究。因此，我们调查了接受辅助生殖治疗(ART)的女性FF中磷脂(PL)、胆固醇酯(CHE)、甘油三酯(TG)和非酯化(NE)脂部分中与BMI相关的FAs浓度。在这项描述性横断面研究中，对接受ART的正常体重(18.5 ≤ BMI <； 25.0 kg/m(2)，n = 10)、超重(25.0 ≤ BMI <； 30.0 kg/m(2)，n = 10)和肥胖(BMI ≥ 30.0 kg/m(2)，n = 10)妇女的FF进行了采样，并用气相色谱分析方法分析了PL、CHE、TG和NEFA部分中23种特定的FA。通过单变量一般线性模型和事后Sheffé检验来研究BMI组之间的差异。不同BMI组间PL和ChE部分的总FA浓度没有差异。BMI组之间的总甘油三酯浓度和总NEFA浓度有显著差异。有趣的是，总脂肪酸的42%和34%分别在PL和CHE部分中酯化，而在TG和NEFA部分中只有10%。在BMI组之间，PL、CHE和TG部分中只有很少的个体FA浓度不同，而在NEFA部分中发现了大量与BMI相关的差异。我们的数据显示，BMI的差异与FF的FA组成的变化有关，这种影响在NEFA部分最为明显。这些与体重指数相关的变异可能影响颗粒细胞活性、卵母细胞发育能力和随后的胚胎质量，这可能解释了其他人描述的肥胖症患者卵母细胞质量的差异。
It has been well documented that the maturing oocyte is very vulnerable to changes in its micro-environment, the follicular fluid (FF). Recent research has focused on different components within this FF, like hormones, growth factors and metabolites, and how their concentrations are altered by diet and the metabolic health of the mother. It has been proposed that fatty acids (FAs) are potential factors that influence oocyte maturation and subsequent embryo development. However, a thorough study of the specific FF FA composition per lipid fraction and how this may be affected by BMI is currently lacking. Therefore, we investigated the BMI-related concentration of FAs in the phospholipid (PL), cholesteryl-ester (CHE), triglyceride (TG) and non-esterified (NE) lipid fraction in the FF of women undergoing assisted reproductive treatment (ART). In this descriptive cross-sectional study, the FF of normal weight (18.5 ≤ BMI < 25.0 kg/m(2), n = 10), overweight (25.0 ≤ BMI < 30.0 kg/m(2), n = 10) and obese (BMI ≥ 30.0 kg/m(2), n = 10) women, undergoing ART, was sampled and analyzed for 23 specific FAs in the PL, CHE, TG and NEFA fraction, using a gas chromatographic analysis method. Differences between BMI-groups were studied by means of univariate general linear models and post hoc Sheffé tests. Total FA concentrations in the PL and CHE fraction did not differ between BMI groups. Total TG concentrations tended to differ and total NEFA concentrations differed significantly between BMI groups. Interestingly, 42% and 34% of the total FAs was esterified in the PL and CHE fraction, respectively, while only 10% were present in both the TG and NEFA fraction. Only few individual FA concentrations differed in the PL, CHE and TG fraction between BMI groups, whereas abundant BMI-related differences were found in the NEFA fraction. Our data show that differences in BMI are associated with alterations in the FA composition of the FF, an effect most pronounced in the NEFA fraction. These BMI-related variations could possibly affect granulosa cell viability, oocyte developmental competence and subsequent embryo quality possibly explaining differences in oocyte quality in obese patients described by others.
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