DNA sequence copy number aberrations associated with histological subtypes and DNA ploidy in gastric carcinoma.
DNA sequence copy number aberrations associated with histological subtypes and DNA ploidy in gastric carcinoma.
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DOI：
10.1111/j.1349-7006.2001.tb01156.x
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发表时间：
2001-07
期刊：
影响因子：
--
通讯作者：
Sasaki K
中科院分区：
文献类型：
--
作者：
Kong G;Oga A;Park CK;Kawauchi S;Furuya T;Sasaki K
我们应用比较基因组杂交技术和激光扫描细胞仪分析了胃癌DNA序列拷贝数畸变（DSCNAs）和DNA倍体，以阐明胃癌基因组畸变与临床病理参数的关系。33例病例中有32例显示一个或多个DSCNA，每个肿瘤的平均数量为11.7。在2p、3q、6p、7p、7q、8q、12p、13q、19q和20q检测到高水平增益。除了8p、9p、12q和20q处的畸变外，肠道型和弥漫型GC中的总体基因组异常和具有基因组畸变的染色体区域的频率相似。DNA异倍体肿瘤中DSCNAs的总数显著高于DNA二倍体肿瘤。我们检测了以组织学亚型、肿瘤位置和DNA倍体状态为特征的基因组畸变：肠型GC中20 q增加以及8p和9p丢失，弥漫型GC中8p和12 q增加，下三分之一GC中20 q增加，DNA非整倍体GC中5q、9p、10q、16 q和18 q丢失。此外，5q丢失与GC中的DNA非整倍体（P=0.0001）或丢失总数（P=0.001）、获得+丢失（P=0.004）和高水平获得（P=0.001）相关。在这些位点中，染色体8p是唯一的。8p的增加在弥漫型GC中更常见，而8p的丢失在肠型GC中更常见。总之，我们描述了5q，8p和20q的染色体区域，这是感兴趣的GC的进一步调查。
We have analyzed DNA sequence copy number aberrations (DSCNAs) and DNA ploidy by using comparative genomic hybridization and laser scanning cytometer in gastric carcinomas (GCs) to elucidate the genomic aberrations in relation to clinicopathological parameters. Thirty‐two out of 33 cases showed one or more DSCNAs with a mean number of 11.7 per tumor. High‐level gains were detected at 2p, 3q, 6p, 7p, 7q, 8q, 12p, 13q, 19q, and 20q. Frequency of gross genomic abnormalities and chromosome regions that have genomic aberrations were similar in both intestinal‐and diffuse‐type GCs, except aberrations at 8p, 9p, 12q, and 20q. The overall number of DSCNAs was significantly greater in DNA aneuploid tumors than that in DNA diploid tumors. We detected genomic aberrations characterized by histological subtype, tumor location, and DNA ploidy status: gain of 20q and losses of 8p and 9p in intestinal‐type GCs, gains of 8p and 12q in diffuse‐type GCs, gain of 20q in the lower third GCs, and loss of 5q, 9p, lOq, 16q, and 18q in DNA aneuploid GCs. Furthermore, 5q loss is associated with DNA aneuploidy (P=0.0001) or the total number of losses (P=0.001), gain+losses (P=0.004), and high‐level gains (P=0.001) in GCs. Among these loci, chromosome 8p was unique. Gain of 8p was more common in diffuse‐type GC, whereas loss of 8p was more frequently detected in intestinal‐type GC. In conclusion, we describe chromosomal regions of 5q, 8p, and 20q, which are of interest for further investigation of GCs.
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影响因子：
13.5
作者：
Kusano, N;Shiraishi, K;Sasaki, K
通讯作者：
Sasaki, K
DOI：
10.1002/cyto.990120502
发表时间：
1991-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
KAMENTSKY, LA;KAMENTSKY, LD
通讯作者：
KAMENTSKY, LD
DOI：
10.1111/apm.1965.64.1.31
发表时间：
1965-01-01
期刊：
ACTA PATHOLOGICA ET MICROBIOLOGICA SCANDINAVICA
影响因子：
--
作者：
LAUREN, P
通讯作者：
LAUREN, P
影响因子：
3.7
作者：
CHER, ML;MACGROGAN, D;JENSEN, RH
通讯作者：
JENSEN, RH
DOI：
10.1007/s101200050053
发表时间：
1998-12-01
期刊：
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association
影响因子：
--
作者：
Ming, Si-Chun
通讯作者：
Ming, Si-Chun