Safety of 222 nm UVC Irradiation to the Surgical Site in a Rabbit Model.
Safety of 222 nm UVC Irradiation to the Surgical Site in a Rabbit Model.
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DOI：
10.1111/php.13620
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发表时间：
2022-11
影响因子：
3.3
通讯作者：
Kuroda, Ryosuke
中科院分区：
文献类型：
--
作者：
Fukui, Tomoaki;Niikura, Takahiro;Oda, Takahiro;Kumabe, Yohei;Nishiaki, Aiko;Kaigome, Rena;Ohashi, Hiroyuki;Sasaki, Masahiro;Igarashi, Tatsushi;Oe, Keisuke;Hamblin, Michael R.;Kuroda, Ryosuke
对于预防手术部位感染（SSI），持续消毒可能有所帮助。短波长紫外线辐射C（UVC）具有高度杀菌性，但显示出细胞毒性。波长为222 nm的UVC辐射到皮肤被认为是安全的，因为它只到达角质层。然而，222 nm照射未覆盖皮肤的手术区域的安全性尚不清楚。本研究的目的是检查222 nm UVC照射兔模型手术野的安全性。五种类型的组织手术暴露和照射与222或254 nm的UVC。对环丁烷嘧啶二聚体（CPD）进行免疫组织学评估，CPD是UVC造成DNA损伤的指标。在所有组织中，254 nm组的CPD阳性细胞率显著高于其他组。222 nm组在脂肪组织中显示出比对照组显著更多的CPD，但在所有其他组织中没有显著差异。在照射后24 h收集的脂肪组织中，254 nm组显示出高于其他组的CPD，而222 nm组已降至对照水平。这些数据表明，222 nm UVC照射可能是安全预防SSI的新方法。为了防止手术部位感染，持续消毒可能会有所帮助。短波长UVC是高度杀菌的，但显示出细胞毒性。波长为222 nm的UVC辐射到皮肤被认为是安全的，因为它只到达角质层。然而，222 nm照射未覆盖皮肤的手术区域的安全性尚不清楚。在这项研究中，五种类型的组织暴露和照射与222 nm或254 nm的UVC。进行DNA损伤的免疫组织学评估。结果表明，222 nm UVC照射可能是一种安全预防手术部位感染的新方法。
For the prevention of surgical site infection (SSI), continuous disinfection could be helpful. Short wavelength ultraviolet radiation C (UVC) is highly bactericidal but shows cytotoxicity. Radiation of UVC with a wavelength of 222 nm to the skin is considered to be safe because it only reaches the stratum corneum. However, the safety of 222 nm irradiation to the surgical field not covered with skin is unknown. The purpose of this study was to examine the safety of 222 nm UVC irradiation on a surgical field in a rabbit model. Five types of tissue were surgically exposed and irradiated with 222 or 254 nm UVC. Immunohistological assessment against cyclobutane pyrimidine dimer (CPD), an index of DNA damage by UVC, was performed. The CPD‐positive cell rate was significantly higher in the 254 nm group than in the other groups in all tissues. A 222 nm group showed significantly more CPD than control in fat tissue, but no significant difference in all other tissues. In fat tissue collected 24 h after irradiation, the 254 nm group showed higher CPD than the other groups, while the 222 nm group had reduced to the control level. These data suggest that 222 nm UVC irradiation could be a new method to safely prevent SSI. To prevent surgical site infection, continuous disinfection could be helpful. Short wavelength UVC is highly bactericidal but shows cytotoxicity. Radiation of UVC with a wavelength of 222 nm to the skin is considered to be safe because it only reaches the stratum corneum. However, the safety of 222 nm irradiation to the surgical field not covered with skin is unknown. In this study, five types of tissue were exposed and irradiated with 222 nm or 254 nm UVC. An immunohistological assessment of DNA damage was performed. The results suggest that 222 nm UVC irradiation could be a new method to safely prevent surgical site infection.
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