Chronic irradiation with 222-nm UVC light induces neither DNA damage nor epidermal lesions in mouse skin, even at high doses.
Chronic irradiation with 222-nm UVC light induces neither DNA damage nor epidermal lesions in mouse skin, even at high doses.
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DOI：
10.1371/journal.pone.0201259
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Nakane A
中科院分区：
文献类型：
--
作者：
Narita K;Asano K;Morimoto Y;Igarashi T;Nakane A
手术部位感染（SSI）是一个重要的临床问题，与手术发病率和死亡率水平的增加有关。手术期间的UVC照射被认为是预防SSI发展的可能策略。254-nm UVC通过在微生物中产生环丁基嘧啶二聚体（CPD）诱导显著水平的DNA损伤。然而，这种效应不仅在微生物中引起，而且在人类细胞中也引起，并且已确定长期暴露于254 nm UVC代表人类健康危害。相比之下，尽管短波长UVC光，特别是222 nm UVC，已被证明引起杀菌作用，单次照射高剂量的222 nm UVC能量已被报道不会诱导哺乳动物细胞中的致突变性或细胞毒性DNA损伤。然而，高剂量222 nm UVC对哺乳动物细胞的慢性照射效果尚未确定。在这项研究中，它被证明，大量的CPD表达细胞诱导后，小鼠的表皮与少量的单次曝光254-nm的UVC，然后不到一半的这些细胞减少在24小时内。慢性254-nm UVC照射揭示了在小鼠皮肤中诱导晒伤和脱屑。组织学分析表明，CPD表达细胞的数量很少，只检测到高剂量的254-nm UVC慢性照射后角化过度的角质层，并显着增生和细胞间水肿也诱导小鼠表皮。相比之下，222 nm UVC光的长期照射不会在小鼠表皮中诱导致突变或细胞毒性作用。这些结果表明，从灯装置（或设备）发射的222纳米UVC光（旨在衰减波长超过230纳米的有害光）代表了减少患者和医院工作人员中SSI发生率的有前途的工具。
Surgical site infections (SSIs) represent an important clinical problem associated with increased levels of surgical morbidity and mortality. UVC irradiation during surgery has been considered to represent a possible strategy to prevent the development of SSI. 254-nm UVC induces marked levels of DNA damage by generating cyclobutyl pyrimidine dimers (CPD) in microorganisms. However, this effect is elicited not only in microorganisms, but also in human cells, and chronic exposure to 254-nm UVC has been established to represent a human health hazard. In contrast, despite short wavelength-UVC light, especially 222-nm UVC, having been demonstrated to elicit a bactericidal effect, single irradiation with a high dose of 222-nm UVC energy has been reported to not induce mutagenic or cytotoxic DNA lesions in mammalian cells. However, the effect of chronic irradiation with a high dose of 222-nm UVC to mammalian cells has not been determined. In this study, it was demonstrated that large numbers of CPD-expressing cells were induced in the epidermis of mice following treatment with a small amount of single exposure 254-nm UVC, and then less than half of these cells reduced within 24 h. Chronic 254-nm UVC irradiation was revealed to induce sunburn and desquamation in mouse skin. Histological analysis demonstrated that small numbers of CPD-expressing cells were detected only in hyperkeratotic stratum corneum after chronic irradiation with a high dose of 254-nm UVC, and that significant hyperplasia and intercellular edema were also induced in the epidermis of mice. In contrast, chronic irradiation with 222-nm UVC light was revealed not to induce mutagenic or cytotoxic effects in the epidermis of mice. These results indicated that 222-nm UVC light emitted from the lamp apparatus (or device), which was designed to attenuate harmful light present in wavelengths of more than 230 nm, represents a promising tool for the reduction of SSI incidence in patients and hospital staff.
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