Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in porcine intestinal epithelial and antigen presenting cells.
Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in porcine intestinal epithelial and antigen presenting cells.
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DOI：
10.1186/1471-2180-14-126
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发表时间：
2014-05-16
期刊：
影响因子：
4.2
通讯作者：
Kitazawa H
中科院分区：
文献类型：
--
作者：
Villena J;Chiba E;Vizoso-Pinto MG;Tomosada Y;Takahashi T;Ishizuka T;Aso H;Salva S;Alvarez S;Kitazawa H
先前的研究结果表明，鼠李糖乳杆菌CRL1505能够增加儿童对肠道病毒感染的抵抗力。然而，参与该益生菌免疫调节作用的肠道细胞、细胞因子和受体尚未被充分表征。我们旨在深入了解CRL1505菌株免疫调节作用的机制，因此在体外评估鼠李糖L. rhamnosus CRL1505与猪肠上皮细胞（IECs）和猪佩尔斑抗原呈递细胞（APCs）之间的串扰，以加深我们对该菌株可能有助于预防病毒性腹泻的机制的了解。L. rhamnosus CRL1505能够在IECs中诱导IFN -α和-β，并在不依赖toll样受体(TLR)-2或TLR9信号的情况下，在poly（I:C）刺激下改善I型IFN的产生。此外，CRL1505菌株通过TLR2诱导IECs中IL-6和TNF-α的mRNA表达。此外，菌株显著提高了肠道APCs的表面分子表达和细胞因子的产生。L. rhamnosus CRL1505诱导的Th1反应的改善是由TLR2信号触发的，包括apc中MHC-II和共刺激分子的表达增加以及IL-1β、IL-6和IFN-γ的表达。IL-10在APCs中也被CRL1505显著上调。最近回顾了TLR激动剂的出现，通过引入比干扰素治疗副作用更小的全病毒治疗来改变抗病毒治疗的新方法。利用鼠李糖CRL1505作为先天免疫调节剂和抗病毒I型IFN、IFN-γ和调节性IL-10的诱导性因子，显然提供了克服这一挑战的潜力。
Previous findings suggested that Lactobacillus rhamnosus CRL1505 is able to increase resistance of children to intestinal viral infections. However, the intestinal cells, cytokines and receptors involved in the immunoregulatory effect of this probiotic strain have not been fully characterized. We aimed to gain insight into the mechanisms involved in the immunomodulatory effect of the CRL1505 strain and therefore evaluated in vitro the crosstalk between L. rhamnosus CRL1505, porcine intestinal epithelial cells (IECs) and antigen presenting cells (APCs) from swine Peyer’s patches in order to deepen our knowledge about the mechanisms, through which this strain may help preventing viral diarrhoea episodes. L. rhamnosus CRL1505 was able to induce IFN–α and –β in IECs and improve the production of type I IFNs in response to poly(I:C) challenge independently of Toll-like receptor (TLR)-2 or TLR9 signalling. In addition, the CRL1505 strain induced mRNA expression of IL-6 and TNF-α via TLR2 in IECs. Furthermore, the strain significantly increased surface molecules expression and cytokine production in intestinal APCs. The improved Th1 response induced by L. rhamnosus CRL1505 was triggered by TLR2 signalling and included augmented expression of MHC-II and co-stimulatory molecules and expression of IL-1β, IL-6, and IFN-γ in APCs. IL-10 was also significantly up-regulated by CRL1505 in APCs. It was recently reviewed the emergence of TLR agonists as new ways to transform antiviral treatments by introducing panviral therapeutics with less adverse effects than IFN therapies. The use of L. rhamnosus CRL1505 as modulator of innate immunity and inductor of antiviral type I IFNs, IFN-γ, and regulatory IL-10 clearly offers the potential to overcome this challenge.
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影响因子：
29.4
作者：
Bass, DM
通讯作者：
Bass, DM
影响因子：
29.4
作者：
Greenberg HB;Estes MK
通讯作者：
Estes MK
影响因子：
2.2
作者：
Liu, Fangning;Li, Guohua;Yuan, Lijuan
通讯作者：
Yuan, Lijuan
影响因子：
4.4
作者：
Christensen, HR;Frokiær, H;Pestka, JJ
通讯作者：
Pestka, JJ
影响因子：
3.1
作者：
Cario, E;Podolsky, DK
通讯作者：
Podolsky, DK