Phylogenetic and recombination analysis of tomato spotted wilt virus.
Phylogenetic and recombination analysis of tomato spotted wilt virus.
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DOI：
10.1371/journal.pone.0063380
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kim KH
中科院分区：
文献类型：
--
作者：
Lian S;Lee JS;Cho WK;Yu J;Kim MK;Choi HS;Kim KH
番茄斑点枯萎病毒（TSWV）严重危害和降低了世界上许多重要经济植物的产量。在这项研究中，我们确定了最近从韩国不同地区和宿主中鉴定出的10株TSWV分离株的全基因组序列。对这10株分离株和之前测序的3株分离株的系统发育分析表明，13株韩国TSWV分离株可分为两组，反映了韩国TSWV分离株的两个不同起源或分化。此外，对13株韩国TSWV分离株的完整核苷酸序列以及先前从韩国和其他国家测序的TSWV RNA片段进行了系统发育和重组分析。系统发育分析表明，大部分韩国分离株的RNA L和RNA M片段可能起源于西欧和北美，但所有韩国分离株的RNA S片段可能起源于中国和日本。重组分析发现，所有分离株和片段共有12个重组事件，13个韩国分离株中有5个重组事件；在韩国发现的5个重组基因中，有3个包含了完整的RNA L片段，这表明是重组而不是重组。我们的分析提供了证据，证明重组和重组都有助于TSWV的分子多样性。
Tomato spotted wilt virus (TSWV) severely damages and reduces the yield of many economically important plants worldwide. In this study, we determined the whole-genome sequences of 10 TSWV isolates recently identified from various regions and hosts in Korea. Phylogenetic analysis of these 10 isolates as well as the three previously sequenced isolates indicated that the 13 Korean TSWV isolates could be divided into two groups reflecting either two different origins or divergences of Korean TSWV isolates. In addition, the complete nucleotide sequences for the 13 Korean TSWV isolates along with previously sequenced TSWV RNA segments from Korea and other countries were subjected to phylogenetic and recombination analysis. The phylogenetic analysis indicated that both the RNA L and RNA M segments of most Korean isolates might have originated in Western Europe and North America but that the RNA S segments for all Korean isolates might have originated in China and Japan. Recombination analysis identified a total of 12 recombination events among all isolates and segments and five recombination events among the 13 Korea isolates; among the five recombinants from Korea, three contained the whole RNA L segment, suggesting reassortment rather than recombination. Our analyses provide evidence that both recombination and reassortment have contributed to the molecular diversity of TSWV.
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