Genetic recombination between RNA components of a multipartite plant virus.
Genetic recombination between RNA components of a multipartite plant virus.
复制标题
多部分植物病毒的RNA成分之间的遗传重组。
作者：
Bujarski JJ;Kaesberg P
DNA的遗传重组是DNA生物进化的基本机制之一，并导致其多样性和适应性。对于大多数植物病毒和许多动物病毒中存在的基于RNA的基因组，重组的重要性远没有那么明显。RNA重组已被证明可促进小核糖核酸病毒的进化变异1 -4，它参与正链和负链病毒缺陷性干扰（DI）RNA的产生5 -9，并参与流感病毒10和冠状病毒11信使RNA的合成。然而，迄今为止，在感染植物的病毒中尚未发现RNA重组。事实上，DI RNA的缺乏和在混合感染的植物中不能证明重组被认为是植物不支持病毒RNA重组的证据。在这里，我们提供了植物病毒RNA重组的第一个分子证据。对于雀麦花叶病毒（BMV），一种单子叶植物的正链、三部分基因组病毒，我们发现在感染发展过程中，单个BMV RNA基因组组分的3′端区域的缺失可以通过与剩余的两种野生型BMV RNA组分中的任一种的同源区域重组来修复。这一结果清楚地表明，植物病毒具有以前被认为仅存在于动物宿主中的强大重组机制，因此它们能够以与动物病毒相当的方式适应和多样化。此外，我们的观察表明，病毒作为载体在植物中引入新的基因的多功能性增加。
Genetic recombination of DNA is one of the fundamental mechanisms underlying the evolution of DNA-based organisms and results in their diversity and adaptability. The importance of the role of recombination is far less evident for the RNA-based genomes that occur in most plant viruses and in many animal viruses. RNA recombination has been shown to promote the evolutionary variation of picornaviruses1–4, it is involved in the creation of defective interfering (DI) RNAs of positive- and negative-strand viruses5–9 and is implicated in the synthesis of the messenger RNAs of influenza virus10 and coronavirus11. However, RNA recombination has not been found to date in viruses that infect plants. In fact, the lack of DI RNAs and the inability to demonstrate recombination in mixedly infected plants has been regarded as evidence that plants do not support recombination of viral RNAs. Here we provide the first molecular evidence for recombination of plant viral RNA. For brome mosaic virus (BMV), a plus-stranded, tripartite-genome virus of monocots, we show that a deletion in the 3′ end region of a single BMV RNA genomic component can be repaired during the development of infection by recombination with the homologous region of either of the two remaining wild-type BMV RNA components. This result clearly shows that plant viruses have available powerful recombinatory mechanisms that previously were thought to exist only in animal hosts, thus they are able to adapt and diversify in a manner comparable to animal viruses. Moreover, our observation suggests an increased versatility of viruses for use as vectors in introducing new genes into plants.
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影响因子：
3.7
作者：
COOPER, PD
通讯作者：
COOPER, PD
DOI：
10.1073/pnas.81.14.4358
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HASELOFF, J;GOELET, P;KAESBERG, P
通讯作者：
KAESBERG, P
影响因子：
64.5
作者：
FIELDS, S;WINTER, G
通讯作者：
WINTER, G
影响因子：
5.4
作者：
KING, AMQ;MCCAHON, D;NEWMAN, JWI
通讯作者：
NEWMAN, JWI
影响因子：
5.3
作者：
OKAYAMA, H;BERG, P
通讯作者：
BERG, P