WT1 Inhibits Human Renal Carcinoma Cell Proliferation and Induces G2/M Arrest by Upregulating IL-24 Expression.
WT1 Inhibits Human Renal Carcinoma Cell Proliferation and Induces G2/M Arrest by Upregulating IL-24 Expression.
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WT1 通过上调 IL-24 表达抑制人肾癌细胞增殖并诱导 G2/M 期阻滞
DOI：
10.1155/2022/1093945
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发表时间：
2022
影响因子：
--
通讯作者：
Chen, Z. H.
中科院分区：
文献类型：
--
作者：
Jing, Y. J.;Lin, L. C.;Chen, L. L.;Huang, Z. E.;Qin, H. C.;Li, S. B.;Chen, Z. H.
The transcription factor Wilms' tumor 1 (WT1) is involved in development, tissue homeostasis, and disease. However, the exact roles and the mechanisms of WT1 in renal carcinoma are not well understood. Therefore, in this study, we evaluated the ability of WT1 to block proliferation in renal carcinoma cells in vitro. Experimental analysis showed that WT1 overexpression inhibited the proliferation of renal carcinoma A498 cells and promoted arrest at the G2/M checkpoint. RNA-Seq identified differentially expressed genes, including IL-24, related to both the cell proliferation and the cell cycle. WT1 overexpression upregulated IL-24 expression, and IL-24 overexpression induced G2/M arrest. ChIP-Seq identified JUN as a direct target of WT1 in A498 cells, in which positive regulation was shown by RT-qPCR. It has been shown that the transcription factor JUN can regulate IL-24 expression, and therefore, we hypothesize that WT1 might regulate the IL-24 through JUN. Furthermore, analysis based on TCGA datasets showed that the expression of WT1-regulated genes, including TXNIP and GADD45A, was significantly correlated with the stage and histological grade of tumors, with high levels linked to favorable prognoses. Our results demonstrated that the overexpression of WT1 upregulates IL-24, leading to G2/M checkpoint arrest to reduce proliferation. These results indicate that regulation of IL-24 by WT1 inhibits proliferation and may represent a potential target for treating renal carcinoma.
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影响因子：
7.4
作者：
Liao L;Zhang L;Yang M;Wang X;Huang W;Wu X;Pan H;Yuan L;Huang W;Wu Y;Guan J
通讯作者：
Guan J
活体动物细胞周期 DNA 合成阶段的生物发光成像
影响因子：
3.7
作者：
Chen ZH;Zhao RJ;Li RH;Guo CP;Zhang GJ
通讯作者：
Zhang GJ
腺病毒介导的人白细胞介素24转染对A549/DDP肺癌细胞的顺铂耐药性的逆转作用。
影响因子：
4.2
作者：
Xu M;Tang X;Guo J;Sun W;Tang F
通讯作者：
Tang F
DOI：
10.1111/j.1349-7006.1999.tb00733.x
发表时间：
1999-03
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Oji Y;Ogawa H;Tamaki H;Oka Y;Tsuboi A;Kim EH;Soma T;Tatekawa T;Kawakami M;Asada M;Kishimoto T;Sugiyama H
通讯作者：
Sugiyama H
TXNIP作为抑癌基因参与多种癌症中癌细胞代谢重编程和氧化应激的研究进展
影响因子：
4.7
作者：
Chen Y;Ning J;Cao W;Wang S;Du T;Jiang J;Feng X;Zhang B
通讯作者：
Zhang B