Comparison between optical coherence tomography angiography and immunolabeling for evaluation of laser-induced choroidal neovascularization.
Comparison between optical coherence tomography angiography and immunolabeling for evaluation of laser-induced choroidal neovascularization.
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DOI：
10.1371/journal.pone.0201958
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Takahashi K
中科院分区：
文献类型：
--
作者：
Nakagawa K;Yamada H;Mori H;Toyama K;Takahashi K
本研究旨在探讨小鼠模型中光学相干断层扫描血管造影 (OCTA) 获得的图像与激光诱导脉络膜新生血管 (CNV) 免疫组织化学标记获得的图像之间的差异。通过激光光凝（GYC-2000，NIDEK；波长 532 nm）在 10 只 6 周龄雌性 C57BL/6J 小鼠的左眼中诱导 CNV。激光参数包括 100 μm 光斑、100 ms 脉冲持续时间和 200 mW 入射功率，以破裂 Bruch 膜。 OCT 和 OCTA CNV 图像是在激光光凝术后 5 天使用 RS-3000 Advance (NIDEK) 获得的。 OCTA 成像后，分离的脉络膜/视网膜色素上皮复合物用 CD31（一种内皮细胞标记物）、血小板衍生生长因子受体 β（PDGFRβ，一种周细胞样支架标记物）、α-平滑肌肌动蛋白 (α-SMA) 和胶原蛋白 I 进行荧光标记。通过 enface OCTA 获得的病变面积测量值与 脉络膜平片中免疫标记的 CD31+ CNV 病变。我们还检查了 PDGFRβ+ 周细胞样支架和 OCTA 图像之间的结构相关性。 Enface OCTA 清楚地检测到激光诱导的 CNV，表现为被暗晕包围的高血流病变。通过免疫标记获得的 CNV 病变面积测量值明显大于通过 enface OCTA 获得的面积测量值 (p = 0.006)。由于暗晕，周细胞样支架外围下方的 CNV 病变通过 Enface OCTA 无法清晰可见；然而，通过横截面 OCTA 可以将病变检测为血流，并且也被 CD31 高度标记。周细胞样支架的外围似乎发展为视网膜下纤维化，并且该区域富含肌成纤维细胞。 Enface OCTA 无法检测小鼠中激光诱导的 CNV 的整个区域，无法检测到的部分位于周细胞样支架的纤维化外围下方。由于这种 OCTA 纤维化伪影，OCTA 成像在准确估计 CNV 病变方面的潜力有限。
This study aimed to investigate the differences between images obtained by optical coherence tomography angiography (OCTA) with those from immunohistochemical labeling of laser-induced choroidal neovascularization (CNV) in a mouse model. CNV was induced by laser photocoagulation (GYC-2000, NIDEK; wavelength 532 nm) in the left eyes of 10 female C57BL/6J mice aged 6 weeks. The laser parameters included a 100-μm spot, 100-ms pulse duration and 200-mW incident power to rupture Bruch’s membrane. OCT and OCTA CNV images were obtained using the RS-3000 Advance (NIDEK) 5 days post-laser photocoagulation. After OCTA imaging, the isolated choroid/retinal pigment epithelium complexes were fluorescently labeled with CD31 (an endothelial cell marker), platelet-derived growth factor receptor β (PDGFRβ, a pericyte-like scaffold marker), α-smooth muscle actin (α-SMA) and collagen I. Area measurements of the lesions obtained by enface OCTA were compared with immunolabeled CD31+ CNV lesions in choroid flat-mounts. We also examined structural correlations between the PDGFRβ+ pericyte-like scaffold and OCTA images. Laser-induced CNV was clearly detected by enface OCTA, appearing as a hyperflow lesion surrounded by a dark halo. Area measurements of the CNV lesion by immunolabeling were significantly larger than those obtained by enface OCTA (p = 0.006). The CNV lesion beneath the periphery of the pericyte-like scaffold was not clearly visible by enface OCTA due to the dark halo; however, the lesion was detectable as blood flow by cross-sectional OCTA and was also highly labeled by CD31. The periphery of the pericyte-like scaffold appeared to develop into subretinal fibrosis and this region was rich in myofibroblasts. Enface OCTA was unable to detect the entire area of laser-induced CNV in mice, with an undetectable portion located beneath the fibrotic periphery of the pericyte-like scaffold. Due to this OCTA fibrosis artifact, OCTA imaging has limited potential for accurately estimating CNV lesions.
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影响因子：
3.8
作者：
Jia Y;Tan O;Tokayer J;Potsaid B;Wang Y;Liu JJ;Kraus MF;Subhash H;Fujimoto JG;Hornegger J;Huang D
通讯作者：
Huang D
影响因子：
4.4
作者：
Costanzo, Eliana;Miere, Alexandra;Souied, Eric H.
通讯作者：
Souied, Eric H.
影响因子：
6
作者：
Strittmatter, Karin;Pomeroy, Hayley;Marneros, Alexander G.
通讯作者：
Marneros, Alexander G.
影响因子：
6
作者：
He, Lizhi;Marneros, Alexander G.
通讯作者：
Marneros, Alexander G.
DOI：
10.1097/iae.0000000000000773
发表时间：
2015-11-01
影响因子：
3.3
作者：
El Ameen, Ala;Cohen, Salomon Y.;Souied, Eric H.
通讯作者：
Souied, Eric H.