Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy.
Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy.
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Medaka Fish，Oryzias latipes，作为与肥胖相关的肾小球病的模型。
DOI：
10.1016/j.bbrc.2013.01.053
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发表时间：
2013-02-22
影响因子：
3.1
通讯作者：
Obara, Tomoko
中科院分区：
文献类型：
--
作者：
Ichimura, Koichiro;Kawashima, Yusuke;Nakamura, Tomomi;Powell, Rebecca;Hidoh, Yuya;Terai, Shuji;Sakaida, Isao;Kodera, Yoshio;Tsuji, Takashi;Ma, Jian-Xing;Sakai, Tatsuo;Matsumoto, Hiroyuki;Obara, Tomoko
肥胖是以代谢综合征为特征的复杂疾病的一部分，是一个持续的重大公共卫生问题。青鳉和斑马鱼是重要的水生实验动物，广泛应用于毒理学和环境卫生科学领域，并作为人类疾病模型。在青鳉中，高脂饮食（HFD）的简单喂养可诱导体重增加、内脏脂肪组织的过度积累、高血糖症、高脂血症和脂肪肝，其模拟人类代谢综合征。在本研究中，探索的可能性，成人青少年喂养HFD（HFD青少年）可作为人类代谢综合征相关的肾小球疾病，包括肥胖相关的肾小球疾病（ORG）的动物模型，我们分析了结构的改变和蛋白质表达的HFD青少年中肾。病理组织学观察发现，肾组织病理学表现为肾小球增大，伴肾小球毛细血管扩张和系膜增生性扩张，与人ORG部分相似，且MYH 9、SM 22 α等多种肾脏疾病相关蛋白表达明显升高。因此，HFD-青鳉具有作为用于探索人类ORG的机制的动物模型的高潜力。
Obesity, an ongoing significant public health problem, is a part of complex disease characterized as metabolic syndrome. Medaka and zebrafish are useful aquatic experimental animals widely used in the field of toxicology and environmental health sciences and as a human disease models. In medaka, simple feeding of a high fat diet (HFD) can induce body weight gain, excessive accumulation of visceral adipose tissue, hyperglycemia, hyperlipidemia, and steatohepatists, which mimics human metabolic syndrome. In the present study, to explore the possibility that the adult medaka fed with HFD (HFD-medaka) can be used as an animal model for human metabolic syndrome-associated glomerular disease, including obesity-related glomerulopathy (ORG), we analyzed structural alterations and protein expression in the mesonephric kidney of HFD-medaka. We found that the histopathology was consistent with glomerulomegaly accompanied by the dilation of glomerular capillaries and proliferative expansion of the mesangium, a condition partially comparable to human ORG. Moreover, expressions of several kinds of kidney disease-related proteins (such as MYH9, SM22α) were significantly elevated. Thus, the HFD-medaka has a high potential as an animal model useful for exploring the mechanism underling human ORG.
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与MyH9相关的肾病的光谱。
DOI：
10.2215/cjn.08721209
发表时间：
2010-06
期刊：
Clinical journal of the American Society of Nephrology : CJASN
影响因子：
--
作者：
Bostrom MA;Freedman BI
通讯作者：
Freedman BI
影响因子：
30.8
作者：
Kopp, Jeffrey B.;Smith, Michael W.;Nelson, George W.;Johnson, Randall C.;Freedman, Barry I.;Bowden, Donald W.;Oleksyk, Taras;McKenzie, Louise M.;Kajiyama, Hiroshi;Ahuja, Tejinder S.;Berns, Jeffrey S.;Briggs, William;Cho, Monique E.;Dart, Richard A.;Kimmel, Paul L.;Korbet, Stephen M.;Michel, Donna M.;Mokrzycki, Michele H.;Schelling, Jeffrey R.;Simon, Eric;Trachtman, Howard;Vlahov, David;Winkler, Cheryl A.
通讯作者：
Winkler, Cheryl A.
DOI：
10.1152/ajprenal.00187.2010
发表时间：
2011-04-01
影响因子：
4.2
作者：
Marshall, Caroline B.;Krofft, Ron D.;Shankland, Stuart J.
通讯作者：
Shankland, Stuart J.
影响因子：
19.6
作者：
Kambham, N;Markowitz, GS;D'Agati, VD
通讯作者：
D'Agati, VD
影响因子：
13.2
作者：
JENNETTE, JC;CHARLES, L;GRUBB, W
通讯作者：
GRUBB, W