SARS-CoV-2 Delta Variant (AY.3) in the Feces of a Domestic Cat.
SARS-CoV-2 Delta Variant (AY.3) in the Feces of a Domestic Cat.
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DOI：
10.3390/v14020421
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发表时间：
2022-02-17
期刊：
影响因子：
--
通讯作者：
Lennon EM
中科院分区：
文献类型：
--
作者：
Lenz OC;Marques AD;Kelly BJ;Rodino KG;Cole SD;Perera RAPM;Weiss SR;Bushman FD;Lennon EM
自全球COVID-19大流行开始以来，严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）感染已从人类蔓延到伴侣和野生动物。然而，感染非人类动物物种的病毒基因组的全基因组测序数据很少。在这里，我们在一只11岁家猫的粪便样本中检测到SARS-CoV-2 δ变体（AY.3）并对其进行了测序，该家猫之前曾接触过SARS-CoV-2检测呈阳性的主人。对相隔7天采集的两个粪便样本进行分子检测，发现病毒RNA水平相对较高。对猫源病毒基因组的测序表明，两者是相同的，与在同一地理区域和同一时期收集的人类源病毒谱系AY.3序列进行两两比较时，存在4到14个单核苷酸多态性的差异。然而，在系统发育分析中，猫科动物样本的一些独特突变揭示了它们与这一群体的差异。这些结果表明，新出现的SARS-CoV-2变体的持续外溢感染威胁着人类和动物的健康，并强调了在动物中检测SARS-CoV-2时收集粪便样本的重要性。据作者所知，这是美国家猫中首次发表的SARS-CoV-2 delta变体病例。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections have spilled over from humans to companion and wild animals since the inception of the global COVID-19 pandemic. However, whole genome sequencing data of the viral genomes that infect non-human animal species have been scant. Here, we detected and sequenced a SARS-CoV-2 delta variant (AY.3) in fecal samples from an 11-year-old domestic house cat previously exposed to an owner who tested positive for SARS-CoV-2. Molecular testing of two fecal samples collected 7 days apart yielded relatively high levels of viral RNA. Sequencing of the feline-derived viral genomes showed the two to be identical, and differing by between 4 and 14 single nucleotide polymorphisms in pairwise comparisons to human-derived lineage AY.3 sequences collected in the same geographic area and time period. However, several mutations unique to the feline samples reveal their divergence from this cohort on phylogenetic analysis. These results demonstrate continued spillover infections of emerging SARS-CoV-2 variants that threaten human and animal health, as well as highlight the importance of collecting fecal samples when testing for SARS-CoV-2 in animals. To the authors’ knowledge, this is the first published case of a SARS-CoV-2 delta variant in a domestic cat in the United States.
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DOI：
10.1126/science.abe5901
发表时间：
2021-01-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
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通讯作者：
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影响因子：
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影响因子：
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影响因子：
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影响因子：
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作者：
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