Postsynaptic odorant concentration dependent inhibition controls temporal properties of spike responses of projection neurons in the moth antennal lobe.
Postsynaptic odorant concentration dependent inhibition controls temporal properties of spike responses of projection neurons in the moth antennal lobe.
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DOI：
10.1371/journal.pone.0089132
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kanzaki R
中科院分区：
文献类型：
--
作者：
Fujiwara T;Kazawa T;Haupt SS;Kanzaki R
虽然嗅觉神经元的气味浓度-反应特性已经在多种动物中得到了广泛的研究，但气味浓度在回路水平上对神经处理的影响仍然知之甚少。利用家蚕触角叶（AL）信息素处理回路的钙显像技术，研究了气味浓度对二级投射神经元（PN）反应的影响。随着气味浓度的增加，树突的PN钙反应单调增加，而在较高的气味浓度下，体细胞的钙反应由于突触后抑制而下降。同时钙成像和电生理显示PN体的钙反应而不是树突反应尖峰活动。抑制作用缩短了尖峰反应持续时间，而不是降低了瞬时尖峰频率。局部中间神经元（LNs）在高气味浓度下被特异性激活，PN反应被抑制，被认为是抑制的来源。我们的研究结果表明，在一个广泛的气味浓度范围内，存在一种保留嗅觉处理时间分辨率的小球粒内机制。
Although odorant concentration-response characteristics of olfactory neurons have been widely investigated in a variety of animal species, the effect of odorant concentration on neural processing at circuit level is still poorly understood. Using calcium imaging in the silkmoth (Bombyx mori) pheromone processing circuit of the antennal lobe (AL), we studied the effect of odorant concentration on second-order projection neuron (PN) responses. While PN calcium responses of dendrites showed monotonic increases with odorant concentration, calcium responses of somata showed decreased responses at higher odorant concentrations due to postsynaptic inhibition. Simultaneous calcium imaging and electrophysiology revealed that calcium responses of PN somata but not dendrites reflect spiking activity. Inhibition shortened spike response duration rather than decreasing peak instantaneous spike frequency (ISF). Local interneurons (LNs) that were specifically activated at high odorant concentrations at which PN responses were suppressed are the putative source of inhibition. Our results imply the existence of an intraglomerular mechanism that preserves time resolution in olfactory processing over a wide odorant concentration range.
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