Enhancing size based size separation through vertical focus microfluidics using secondary flow in a ridged microchannel.
Enhancing size based size separation through vertical focus microfluidics using secondary flow in a ridged microchannel.
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DOI：
10.1038/s41598-017-17388-w
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发表时间：
2017-12-12
影响因子：
4.6
通讯作者：
Sulchek T
中科院分区：
文献类型：
--
作者：
Tasadduq B;Lam W;Alexeev A;Sarioglu AF;Sulchek T
生物颗粒和细胞的基于尺寸的高通量分离和分选对于用于医学诊断和药物纯化的各种生物医学处理步骤是至关重要的。改进基于微流体尺寸的颗粒/细胞分选是一个挑战，以更好地解决产生更均匀的亚群用于研究和使用的需要。我们提出了一种新的进步，微流体分选装置，使用三维聚焦的样品，以最佳的位置颗粒放大的大小依赖的差异所造成的不同的二次流的轨迹。结果是与未聚焦流相比，小颗粒的纯度增加了35倍，大颗粒的纯度增加了8倍。我们的模拟和实验数据首次揭示了在三维空间中定位颗粒可以用来更好地利用不同尺寸的颗粒在具有横向二次流的微通道中流动时的差分横向运动。聚焦方法也可用于改善具有多个平衡位置的惯性通道的颗粒的定位。该技术可在一分钟内对数百万个颗粒和细胞进行连续流、高通量的基于尺寸的分选，无需任何预处理和后处理。我们还证明了从人血液中富集和回收白色血细胞的改进。
High throughput size based separation and sorting of bioparticles and cells is critical to a variety of biomedical processing steps for medical diagnostics and pharmaceutical purification. Improving microfluidic size-based particle/cell sorting is a challenge to better address the need for generating more homogeneous subpopulations for study and use. We propose a novel advance to microfluidic sorting devices that uses three-dimensional focusing of the sample to optimally position particles to amplify the size-dependent differences in trajectories caused by differential secondary flows. The result is an increase in the purity of small particles by 35- fold and large particles by 8-fold in comparison to unfocused flow. Our simulated and experimental data reveal for the first time that positioning particles in three-dimensional space can be used to better leverage the differential lateral movement of particles with different sizes as they flow in microchannel with transverse secondary flows. The focusing approach may also be useful to improve positioning of particles with inertial channels with multiple equilibrium positions. This technique performs continuous-flow, high throughput size based sorting of millions of particles and cells in a minute without any pre and post-processing. We have also demonstrated improved enrichment and recovery of white blood cells from human blood.
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影响因子：
6.1
作者：
Choi, Sungyoung;Park, Je-Kyun
通讯作者：
Park, Je-Kyun
影响因子：
6.1
作者：
Choi, Sungyoung;Park, Je-Kyun
通讯作者：
Park, Je-Kyun
DOI：
10.1111/j.1751-2824.2008.00195.x
发表时间：
2008-01-01
期刊：
ISBT SCIENCE SERIES, VOL 3, NO 2: INTRODUCTION TO BLOOD TRANSFUSION TECHNOLOGY
影响因子：
--
作者：
Hardwick, J.
通讯作者：
Hardwick, J.
DOI：
10.1182/blood.v33.2.353.353
发表时间：
1969-01-01
期刊：
BLOOD-THE JOURNAL OF HEMATOLOGY
影响因子：
--
作者：
BUCKNER, D;GRAW, RG;PERRY, S
通讯作者：
PERRY, S
影响因子：
1.6
作者：
BONNER, WA;SWEET, RG;HERZENBERG, LA
通讯作者：
HERZENBERG, LA