Characterisation of the antiviral RNA interference response to Toscana virus in sand fly cells.
Characterisation of the antiviral RNA interference response to Toscana virus in sand fly cells.
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DOI：
10.1371/journal.ppat.1011283
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发表时间：
2023-03
期刊：
影响因子：
6.7
通讯作者：
中科院分区：
文献类型：
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托斯卡纳病毒（TOSV）（布尼亚病毒目，Phenuiviridae，Phlebovirus，托斯卡纳Phlebovirus）和其它相关的人类病原虫媒病毒由白蛉传播。据报道，在地中海沿岸的国家以及其他地区都有TOSV的报告。感染可导致发热性疾病以及脑膜炎和脑炎。了解载体-虫媒病毒相互作用对于提高我们对虫媒病毒如何传播的认识至关重要，在这种情况下，控制病毒复制的免疫反应起着重要作用。蚊子媒介对虫媒病毒的免疫已经进行了广泛的研究，其中RNA干扰（RNAi），特别是外源siRNA（exo-siRNA）途径起着关键作用。然而，白蛉的抗病毒免疫力还不太清楚。在这里，我们能够表明exo-siRNA途径在木瓜白蛉衍生的细胞系中是活跃的。在TOSV感染后，检测到独特的21个核苷酸的病毒衍生的小干扰RNA（vsiRNA）。我们还鉴定了该细胞系中的exo-siRNA效应子Ago 2，并且沉默其表达使得exo-siRNA途径基本上失活。因此，我们的数据表明，这一途径是积极的作为一种抗病毒反应，对白蛉传播的布尼亚病毒，TOSV。托斯卡纳病毒（TOSV）是一种布尼亚病毒，通过白蛉叮咬传播给人类。检查控制虫媒病毒的免疫反应对于了解这些生物体如何控制感染至关重要，因为这可能会影响传播。外源性siRNA（exo-siRNA）通路是蚊子体内重要的抗病毒免疫应答途径，但对白蛉中的通路知之甚少。在这里，我们表明，一个功能exo-siRNA途径存在于白蛉衍生的细胞系，它是积极的响应TOSV感染。这些发现提高了我们对一般媒介的抗病毒免疫的理解，特别是白蛉。
Toscana virus (TOSV) (Bunyavirales, Phenuiviridae, Phlebovirus, Toscana phlebovirus) and other related human pathogenic arboviruses are transmitted by phlebotomine sand flies. TOSV has been reported in nations bordering the Mediterranean Sea among other regions. Infection can result in febrile illness as well as meningitis and encephalitis. Understanding vector-arbovirus interactions is crucial to improving our knowledge of how arboviruses spread, and in this context, immune responses that control viral replication play a significant role. Extensive research has been conducted on mosquito vector immunity against arboviruses, with RNA interference (RNAi) and specifically the exogenous siRNA (exo-siRNA) pathway playing a critical role. However, the antiviral immunity of phlebotomine sand flies is less well understood. Here we were able to show that the exo-siRNA pathway is active in a Phlebotomus papatasi-derived cell line. Following TOSV infection, distinctive 21 nucleotide virus-derived small interfering RNAs (vsiRNAs) were detected. We also identified the exo-siRNA effector Ago2 in this cell line, and silencing its expression rendered the exo-siRNA pathway largely inactive. Thus, our data show that this pathway is active as an antiviral response against a sand fly transmitted bunyavirus, TOSV. Toscana virus (TOSV) is a bunyavirus that is transmitted to humans through the bite of phlebotomine sand flies. Examining the immune responses that control arboviruses in their vectors is critical for understanding how these organisms control infection, as this may affect transmission. The exogenous siRNA (exo-siRNA) pathway is a critical antiviral immune response in mosquitoes, but much less is known about the pathway in sand flies. Here we show that a functional exo-siRNA pathway exists in a sand fly-derived cell line and that it is active as a response to TOSV infection. These findings improve our understanding of antiviral immunity in vectors in general, and phlebotomine sand flies in particular.
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影响因子：
6.7
作者：
Gestuveo RJ;Parry R;Dickson LB;Lequime S;Sreenu VB;Arnold MJ;Khromykh AA;Schnettler E;Lambrechts L;Varjak M;Kohl A
通讯作者：
Kohl A
影响因子：
5.9
作者：
Elliott, Richard M.;Brennan, Benjamin
通讯作者：
Brennan, Benjamin
影响因子：
4.4
作者：
Petrella V;Aceto S;Musacchia F;Colonna V;Robinson M;Benes V;Cicotti G;Bongiorno G;Gradoni L;Volf P;Salvemini M
通讯作者：
Salvemini M
影响因子：
3.8
作者：
Varjak M;Donald CL;Mottram TJ;Sreenu VB;Merits A;Maringer K;Schnettler E;Kohl A
通讯作者：
Kohl A
DOI：
10.3390/v7020820
发表时间：
2015-02-17
期刊：
Viruses
影响因子：
--
作者：
Blair CD;Olson KE
通讯作者：
Olson KE