Hydrogen production from butyrate by a marine mixed phototrophic bacterial consort
Hydrogen production from butyrate by a marine mixed phototrophic bacterial consort
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海洋混合光养细菌从丁酸盐生产氢气
DOI：
10.1016/j.ijhydene.2011.11.101
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发表时间：
2012-03
影响因子：
7.2
通讯作者：
Pan, Guanghua
中科院分区：
文献类型：
--
作者：
Cai, Jinling;Wang, Guangce;Pan, Guanghua
以丁酸为唯一碳源，研究了一株新富集的海洋光合细菌共生体的产氢能力。变性梯度凝胶电泳（DGGE）分析表明，混合细菌配偶主要由外硫红螺旋体，孢子乳杆菌，和Rhodovulum。研究的重要参数包括温度、光强度、初始pH和丁酸盐浓度。随着发酵的进行，培养基的pH显著增加。在温度25-35 °C、光强80-120 μmol光子/m2 s、初始pH 8、丁酸盐浓度20-40 mmol/l的条件下观察到最佳细胞生长。最佳产氢条件为30 °C，光照强度80 μmol photons/m2 s，初始pH 8。丁酸浓度增加（10-50 mmol/l），产氢量增加，但产氢率（mol H2/mol丁酸）随丁酸浓度增加而逐渐降低。最大产氢量和产氢速率分别为2.52 ± 0.12 mol H_2/mol丁酸和19.40 ± 2.32 ml/l h。这些结果表明，培养条件的优化导致生物氢产量和细胞生长的同时增加。
A newly enriched marine phototrophic bacterial consort was studied for its capability of hydrogen production in batch cultivations using butyrate as the sole carbon source. Analyses of denaturing gradient gel electrophoresis (DGGE) profiles showed that the mixed bacterial consort consisted mainly of Ectothiorhodospira, Sporolactobacillus, and Rhodovulum. Important parameters investigated include temperature, light intensity, initial pH, and butyrate concentration. The pH of the culture medium significantly increased as fermentation proceeded. Optimal cell growth was observed at temperature of 25–35 °C, light intensity of 80–120 μmol photons/m2s, initial pH of 8, butyrate concentration of 20–40 mmol/l. Optimal conditions for hydrogen production were 30 °C, light intensity of 80 μmol photons/m2s, initial pH 8. The increase of butyrate concentration (10–50 mmol/l) resulted in higher hydrogen production, but the yield of hydrogen production (mol H2/mol butyrate) gradually decreased with increasing butyrate concentration. The maximal hydrogen yield and hydrogen production rate were estimated to be 2.52 ± 0.12 mol H2/mol butyrate and 19.40 ± 2.32 ml/l h, respectively. These results indicate that optimization of the culture conditions resulted in a simultaneous increase in biohydrogen production and cell growth.
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影响因子：
7.2
作者：
Ru Ying Li;H. H. Fang-H.
通讯作者：
Ru Ying Li;H. H. Fang-H.
影响因子：
4.1
作者：
Basak, Nitai;Das, Debabrata
通讯作者：
Das, Debabrata
影响因子：
7.2
作者：
Liu, Bing-Feng;Ren, Nan-Qi;Xie, Guo-Jun
通讯作者：
Xie, Guo-Jun
DOI：
10.1016/0140-6701(96)88586-9
发表时间：
1996-05
期刊：
Fuel and Energy Abstracts
影响因子：
--
作者：
Y. Miura
通讯作者：
Y. Miura
DOI：
10.1016/b978-0-444-56352-1.00008-8
发表时间：
2013
期刊：
--
影响因子：
--
作者：
V. Martínez-Merino;M. Gil;A. Cornejo
通讯作者：
V. Martínez-Merino;M. Gil;A. Cornejo