Effect of cadmium on mRNA mistranslation in Saccharomyces cerevisiae
Effect of cadmium on mRNA mistranslation in Saccharomyces cerevisiae
复制标题
镉对酿酒酵母 mRNA 误译的影响
DOI：
10.1002/jobm.201900495
复制
发表时间：
2020-01
影响因子：
3.1
通讯作者：
Huang Zhiwei
中科院分区：
文献类型：
--
作者：
Zhang Xiaoyu;Kuang Xin;Cao Fangqi;Chen Ranran;Fang Zhijia;Liu Wenbin;Shi Ping;Wang H;ong;Shen Yuhu;Huang Zhiwei
虽然生物体使用高度精确的分子过程和各种信使RNA（mRNA）质量控制和核糖体校对机制来转录其基因并保持遗传信息的保真度，但错误是所有生物系统所固有的。由胁迫条件引起的同源和非同源氨基酸不平衡引起的低水平翻译错误特别常见。有利的表型多样性可以通过真核生物中的这种错误通过未知的分子过程产生。在这里，我们发现显著的镉抗性表型与酿酒酵母中mRNA的错误翻译率增加相关。这种表型变化也与内源含硫氨基酸饥饿有关。与对照组相比，镉引起的误译率显著增加（p <0.01）。随着培养基中半胱氨酸含量的增加，WT（BY4742a）的误译率显著降低（p <0.01）。这表明镉处理和含硫氨基酸饥饿都能引起翻译错误。虽然镉的摄取不依赖于Sul1转运蛋白，但镉诱导的mRNA错误翻译依赖于Sul1p转运蛋白的硫酸盐摄取。此外，镉诱导的翻译错误取决于蛋氨酸的生物合成。总之，镉引起内源性硫饥饿，导致mRNA误译增加，这有助于酵母细胞对镉的抗性。我们提供了一个新的途径介导镉的毒性，我们建议，改变mRNA的错误翻译可能描绘一种不同形式的环境适应。
Although highly accurate molecular processes and various messenger RNA (mRNA) quality control and ribosome proofreading mechanisms are used by organisms to transcribe their genes and maintain the fidelity of genetic information, errors are inherent in all biological systems. Low‐level translation errors caused by an imbalance of homologous and nonhomologous amino acids caused by stress conditions are particularly common. Paradoxically, advantageous phenotypic diversity can be generated by such errors in eukaryotes through unknown molecular processes. Here, we found that the significant cadmium‐resistant phenotype was correlated with an increased mistranslation rate of the mRNA in Saccharomyces cerevisiae. This phenotypic change was also related to endogenous sulfur amino acid starvation. Compared with the control, the mistranslation rate caused by cadmium was significantly increased (p < .01). With the increase of cysteine contents in medium, the mistranslation rate of WT(BY4742a) decreased significantly (p < .01). This demonstrates that cadmium treatment and sulfur amino acid starvation both can induce translation errors. Although cadmium uptake is independent of the Sul1 transporter, cadmium‐induced mRNA mistranslation is dependent on the sulfate uptake of the Sul1p transporter. Furthermore, cadmium‐induced translation errors depend on methionine biosynthesis. Taken together, cadmium causes endogenous sulfur starvation, leading to an increase in the mRNA mistranslation, which contributes to the resistance of yeast cells to cadmium. We provide a new pathway mediating the toxicity of cadmium, and we propose that altering mRNA mistranslation may portray a different form of environmental adaptation.
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影响因子：
7.4
作者：
Cobbett, CS
通讯作者：
Cobbett, CS
DOI：
10.1016/j.biocel.2008.05.007
发表时间：
2008-01-01
影响因子：
4
作者：
Leves, Fiona P.;Tierney, M. Louise;Howitt, Susan M.
通讯作者：
Howitt, Susan M.
影响因子：
5.8
作者：
Huang, Yifan;Zhu, Zhengbo;Cui, Jin
通讯作者：
Cui, Jin
DOI：
10.1073/pnas.0809179105
发表时间：
2008-10-28
影响因子：
11.1
作者：
Ruan, Benfang;Palioura, Sotiria;Soell, Dieter
通讯作者：
Soell, Dieter
影响因子：
11.4
作者：
Silva, Raquel M.;Paredes, Joao A.;Santos, Manuel As
通讯作者：
Santos, Manuel As