Noninvasive temporal detection of early retinal vascular changes during diabetes.
Noninvasive temporal detection of early retinal vascular changes during diabetes.
复制标题
糖尿病早期视网膜血管改变的无创时间检测。
DOI：
10.1038/s41598-020-73486-2
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发表时间：
2020-10-15
影响因子：
4.6
通讯作者：
Sheibani N
中科院分区：
文献类型：
--
作者：
Saghiri MA;Suscha A;Wang S;Saghiri AM;Sorenson CM;Sheibani N
糖尿病相关并发症，包括糖尿病视网膜病变和视力丧失，是主要的健康问题。糖尿病早期视网膜血管改变的检测还没有很好的记录，只有很少的研究涉及这一领域。本研究的目的是使用糖尿病临床前模型的眼底图像非侵入性地评估糖尿病非常早期阶段视网膜血管的时间变化。使用Micron III成像系统对具有不同糖尿病持续时间的非糖尿病和秋田/+雄性小鼠进行眼底成像。图像是从4周龄（糖尿病发作）、8周龄、16周龄和24周龄雄性秋田/+和非糖尿病小鼠获得的。在总共104个眼底图像进行各种特征提取的分析。利用ImageJ中Canny边缘检测器和血管生成分析仪插件的组合来量化眼底图像中的各种视网膜血管变化。进行统计分析以确定从糖尿病和非糖尿病动物的眼底图像中提取的各种特征的显著差异。我们的新图像分析方法导致提取超过20个特征。这些结果表明，其中一些特征在糖尿病持续时间较短的情况下发生了显着变化，而另一些特征在糖尿病持续时间较长的情况下保持不变但发生了变化。这些模式可能区分与糖尿病相关的急性（保护性）和慢性（损害性）变化。我们表明，各种堵塞的组合，可以提取超过20个功能，从视网膜血管眼底图像。这些特征在糖尿病期间发生变化，从而允许定量视网膜血管结构的质量作为疾病进展的生物标志物。此外，我们的方法能够识别不同糖尿病持续时间的糖尿病小鼠之间的独特差异。在糖尿病期间非侵入性检测颞视网膜血管变化的能力可能导致识别在糖尿病介导的微血管变化的发展和进展中重要的特异性标志物，评估治疗干预措施，并最终逆转这些变化以阻止或延迟疾病进展。
Diabetes associated complications, including diabetic retinopathy and loss of vision, are major health concerns. Detecting early retinal vascular changes during diabetes is not well documented, and only few studies have addressed this domain. The purpose of this study was to noninvasively evaluate temporal changes in retinal vasculature at very early stages of diabetes using fundus images from preclinical models of diabetes. Non-diabetic and Akita/+ male mice with different duration of diabetes were subjected to fundus imaging using a Micron III imaging system. The images were obtained from 4 weeks- (onset of diabetes), 8 weeks-, 16 weeks-, and 24 weeks-old male Akita/+ and non-diabetic mice. In total 104 fundus images were subjected to analysis for various feature extractions. A combination of Canny Edge Detector and Angiogenesis Analyzer plug-ins in ImageJ were utilized to quantify various retinal vascular changes in fundus images. Statistical analyses were conducted to determine significant differences in the various extracted features from fundus images of diabetic and non-diabetic animals. Our novel image analysis method led to extraction of over 20 features. These results indicated that some of these features were significantly changed with a short duration of diabetes, and others remained the same but changed after longer duration of diabetes. These patterns likely distinguish acute (protective) and chronic (damaging) associated changes with diabetes. We show that with a combination of various plugging one can extract over 20 features from retinal vasculature fundus images. These features change during diabetes, thus allowing the quantification of quality of retinal vascular architecture as biomarkers for disease progression. In addition, our method was able to identify unique differences among diabetic mice with different duration of diabetes. The ability to noninvasively detect temporal retinal vascular changes during diabetes could lead to identification of specific markers important in the development and progression of diabetes mediated-microvascular changes, evaluation of therapeutic interventions, and eventual reversal of these changes in order to stop or delay disease progression.
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影响因子：
16.2
作者：
Cheung, Ning;Donaghue, Kim C.;Liew, Gerald;Rogers, Sophie L.;Wang, Jie Jin;Lim, Shueh-Wen;Jenkins, Alicia J.;Hsu, Wynne;Lee, Mong Li;Wong, Tien Y.
通讯作者：
Wong, Tien Y.
影响因子：
4.4
作者：
Feit-Leichman, RA;Kinouchi, R;Chen, DF
通讯作者：
Chen, DF
影响因子：
7.7
作者：
Gubitosi-Klug, Rose A.;Talahalli, Ramaprasad;Kern, Timothy S.
通讯作者：
Kern, Timothy S.
影响因子：
3.1
作者：
Fischer, Michael J. M.;Uchida, Sae;Messlinger, Karl
通讯作者：
Messlinger, Karl
DOI：
10.1039/c6an02603f
发表时间：
2017-03-27
期刊：
The Analyst
影响因子：
--
作者：
Aboualizadeh E;Ranji M;Sorenson CM;Sepehr R;Sheibani N;Hirschmugl CJ
通讯作者：
Hirschmugl CJ