Isometric skeletal muscle contractile properties in common strains of male laboratory mice.
Isometric skeletal muscle contractile properties in common strains of male laboratory mice.
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DOI：
10.3389/fphys.2022.937132
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发表时间：
2022
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
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在小鼠模型中评估骨骼肌的收缩功能是研究人员用来测量遗传操作、药物疗效或其他治疗干预的影响的常用实验室技术。经常被忽视的是小鼠的应变影响骨骼肌的功能特性的潜力。因此，我们试图描述各种小鼠品系中后肢肌肉中通常评估的等长力测量的特征。使用6-8周龄雄性小鼠，我们测量了C57 BL/6 NJ，BALB/cJ，FVB/NJ，C57 BL/6 J和C57 BL/10小鼠趾长伸肌（EDL）和比目鱼肌的等长肌力，疲劳敏感性，松弛动力学，肌肉质量，肌纤维横截面积和纤维类型组成。这些数据表明，在小鼠的各种遗传背景下，两种肌肉之间既有独特的差异，也有许多相似之处。比目鱼肌比力（即，每单位尺寸的力）在应变之间表现出更高的变化，而EDL肌肉的比力表现出最小的变化。相比之下，绝对力仅在少数小鼠品系中存在差异，而肌肉形态分析显示，在所有组之间进行比较时，存在许多差异。总的来说，这些数据表明，小鼠的品系可能会影响测量的生物学结果，并可能促进与任何遗传操作或治疗干预的协同效应。因此，研究者必须仔细考虑实验设计中使用的小鼠的遗传背景，并准确记录出版期间使用的小鼠品系。
Assessing contractile function of skeletal muscle in murine models is a commonly employed laboratory technique that investigators utilize to measure the impact of genetic manipulations, drug efficacy, or other therapeutic interventions. Often overlooked is the potential for the strain of the mouse to influence the functional properties of the skeletal muscle. Thus, we sought to characterize commonly assessed isometric force measures in the hindlimb muscles across a variety of mouse strains. Using 6-8-week-old male mice, we measured isometric force, fatigue susceptibility, relaxation kinetics, muscle mass, myofiber cross-sectional area, and fiber type composition of the extensor digitorum longus (EDL) and soleus muscles in C57BL/6NJ, BALB/cJ, FVB/NJ, C57BL/6J, and C57BL/10 mice. The data demonstrate both unique differences and a number of similarities between both muscles in the various genetic backgrounds of mice. Soleus muscle specific force (i.e., force per unit size) exhibited higher variation across strains while specific force of the EDL muscle exhibited minimal variation. In contrast, absolute force differed only in a few mouse strains whereas analysis of muscle morphology revealed many distinctions when compared across all the groups. Collectively, the data suggest that the strain of the mouse can potentially influence the measured biological outcome and may possibly promote a synergistic effect with any genetic manipulation or therapeutic intervention. Thus, it is critical for the investigator to carefully consider the genetic background of the mouse used in the experimental design and precisely document the strain of mouse employed during publication.
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DOI：
10.1007/978-1-4939-3810-0_20
发表时间：
2016
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Iyer SR;Valencia AP;Hernández-Ochoa EO;Lovering RM
通讯作者：
Lovering RM
影响因子：
4.1
作者：
Collins, Brittany C.;Laakkonen, Eija K.;Lowe, Dawn A.
通讯作者：
Lowe, Dawn A.
影响因子：
29
作者：
Parks BW;Sallam T;Mehrabian M;Psychogios N;Hui ST;Norheim F;Castellani LW;Rau CD;Pan C;Phun J;Zhou Z;Yang WP;Neuhaus I;Gargalovic PS;Kirchgessner TG;Graham M;Lee R;Tontonoz P;Gerszten RE;Hevener AL;Lusis AJ
通讯作者：
Lusis AJ
影响因子：
--
作者：
Rau, Christoph D.;Wang, Jessica;Lusis, Aldons J.
通讯作者：
Lusis, Aldons J.
影响因子：
3.7
作者：
Calyjur, Priscila Clara;Almeida, Camila de Freitas;Vainzof, Mariz
通讯作者：
Vainzof, Mariz