Brief Communication: Histological Assessment of Nonhuman Primate Brown Adipose Tissue Highlights the Importance of Sympathetic Innervation.
Brief Communication: Histological Assessment of Nonhuman Primate Brown Adipose Tissue Highlights the Importance of Sympathetic Innervation.
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简短交流：非人类灵长类动物棕色脂肪组织的组织学评估突出了交感神经的重要性。
作者：
本研究的目的是从功能上分析非人灵长类动物棕色脂肪组织（BAT）的关键组织学特征与临床代谢特征的相关性。 腋窝脂肪组织活检标本采集自代谢多样的非人灵长类动物队列，并具有临床代谢相关数据。酪氨酸羟化酶（TH）、解偶联蛋白1（UCP 1）、分化簇31（CD 31）、细胞色素c氧化酶亚基4（考克斯IV）、β 3肾上腺素能受体（β3-AR）的表达和脂肪细胞大小通过免疫组织化学分析进行定量。进行计算机断层扫描以评估身体成分。 酪氨酸羟化酶与BAT样本的全身脂肪质量（作为体重的百分比）呈负相关（p = 0.004），与UCP 1（p = 0.02）、考克斯IV（p = 0.006）、CD 31（p = 0.007）和细胞密度（p = 0.02）呈正相关。BAT中β-3肾上腺素能受体丰度与考克斯IV呈弱正相关（p = 0.04），但与UCP 1或TH表达无显著相关性。 我们的研究结果强调，有一个差距，在神经支配提供给BAT的基础上，身体组成，如TH，神经支配的标志物，和肥胖之间的负相关。这些发现也支持神经支配在BAT功能中的重要性，因为TH丰度不仅支持更瘦的身体组成，而且与BAT中的已知结构元件（UCP 1、考克斯IV、CD 31和细胞密度）正相关。根据我们的观察，β3-AR丰度并不强烈驱动这些结构元件或TH，所有这些都已知在棕色脂肪组织的功能中是重要的。实际上，虽然其他受体（如β2-AR）的作用应在BAT功能中进行审查，但这些结果支持开发安全的交感神经系统兴奋剂，以激活棕色脂肪组织用于肥胖治疗。
The objective of this study was to functionally analyze the correlation of key histological features in brown adipose tissue (BAT) with clinical metabolic traits in nonhuman primates. Axillary adipose tissue biopsies were collected from a metabolically diverse nonhuman primate cohort with clinical metabolism-related data. Expression of tyrosine hydroxylase (TH), uncoupling protein 1 (UCP1), cluster of differentiation 31 (CD31), cytochrome c oxidase subunit 4 (COX IV), beta-3 adrenergic receptor (β3-AR), and adipose cell size were quantified by immunohistochemical analysis. Computed tomography scans were performed to assess body composition. Tyrosine hydroxylase was negatively correlated with whole body fat mass as a percentage of body weight (p = 0.004) and was positively correlated with the density of UCP1 (p = 0.02), COX IV (p = 0.006), CD31 (p = 0.007), and cell density (p = 0.02) of the BAT samples. Beta-3 adrenergic receptor abundance had a weak positive correlation with COX IV (p = 0.04) in BAT but did not significantly correlate to UCP1 or TH expression in BAT. Our findings highlight that there is a disparity in innervation provided to BAT based on body composition, as seen with the negative association between TH, a marker for innervation, and adiposity. These findings also support the importance of innervation in the functionality of BAT, as TH abundance not only supports leaner body composition but is also positively correlated with known structural elements in BAT (UCP1, COX IV, CD31, and cell density). Based on our observations, β3-AR abundance does not strongly drive these structural elements or TH, all of which are known to be important in the function of brown adipose tissue. In effect, while the role of other receptors, such as β2-AR, should be reviewed in BAT function, these results support the development of safe sympathetic nervous system stimulants to activate brown adipose tissue for obesity treatment.
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DOI：
10.1016/j.numecd.2012.01.013
发表时间：
2013-06-01
影响因子：
3.9
作者：
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通讯作者：
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影响因子：
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DOI：
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发表时间：
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影响因子：
11.1
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DOI：
10.1016/j.ijrobp.2019.11.034
发表时间：
2020-03-15
影响因子：
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作者：
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DOI：
10.1073/pnas.1207911109
发表时间：
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影响因子：
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作者：
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