Chemogenetic activation of endogenous arginine vasopressin exerts anorexigenic effects via central nesfatin-1/NucB2 pathway.
Chemogenetic activation of endogenous arginine vasopressin exerts anorexigenic effects via central nesfatin-1/NucB2 pathway.
复制标题
DOI：
10.1186/s12576-021-00802-4
复制
发表时间：
2021-06-16
影响因子：
2.3
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Sanada, Kenya;Yoshimura, Mitsuhiro;Ikeda, Naofumi;Baba, Kazuhiko;Nishimura, Haruki;Nishimura, Kazuaki;Nonaka, Yuki;Maruyama, Takashi;Miyamoto, Tetsu;Mori, Masatomo;Conway-Campbell, Becky;Lightman, Stafford;Kataoka, Masaharu;Ueta, Yoichi
我们研究了内源性精氨酸加压素（AVP）的化学发生激活是否会影响中枢nesfatin-1/NucB 2神经元，使用先前生成的转基因大鼠系。在成年雄性AVP-hM 3Dq-mCherry转基因大鼠（300-370 g）中皮下施用盐水（1 mL/kg）或氯氮平-N-氧化物（CNO，1 mg/mL/kg）（hM 3Dq的激动剂）。皮下（s.c.）注射CNO，昼夜节律异常。s.c.后120 min，下丘脑和脑干nesfatin-1/NucB 2免疫反应阳性神经元Fos表达的百分比显著增加。注射CNO。与载体相比，脑室内施用nesfatin-1/NucB 2-中和抗体后，消除了由内源性AVP的化学发生激活诱导的抑制的食物摄入，而没有任何水摄入或昼夜节律的改变。这些结果表明，内源性AVP的化学发生激活影响，至少部分，中枢nesfatin-1/NucB 2神经元，并可能在转基因大鼠中发挥促凋亡作用。在线版本包含补充材料，可通过10.1186/s12576-021-00802-4获得。
We examined whether the chemogenetic activation of endogenous arginine vasopressin (AVP) affects central nesfatin-1/NucB2 neurons, using a transgenic rat line that was previously generated. Saline (1 mL/kg) or clozapine-N-oxide (CNO, 1 mg/mL/kg), an agonist for hM3Dq, was subcutaneously administered in adult male AVP-hM3Dq-mCherry transgenic rats (300–370 g). Food and water intake were significantly suppressed after subcutaneous (s.c.) injection of CNO, with aberrant circadian rhythmicity. The percentages of Fos expression in nesfatin-1/NucB2-immunoreactive neurons were significantly increased in the hypothalamus and brainstem at 120 min after s.c. injection of CNO. Suppressed food intake that was induced by chemogenetic activation of endogenous AVP was ablated after intracerebroventricularly administered nesfatin-1/NucB2-neutralizing antibody in comparison with vehicle, without any alteration of water intake nor circadian rhythmicity. These results suggest that chemogenetic activation of endogenous AVP affects, at least in part, central nesfatin-1/NucB2 neurons and may exert anorexigenic effects in the transgenic rats. The online version contains supplementary material available at 10.1186/s12576-021-00802-4.
登录
查看更多内容
DOI：
10.1073/pnas.0700293104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Armbruster, Blaine N.;Li, Xiang;Roth, Bryan L.
通讯作者：
Roth, Bryan L.
影响因子：
3
作者：
Yoshimura M;Conway-Campbell B;Ueta Y
通讯作者：
Ueta Y
影响因子：
29
作者：
Maejima, Yuko;Sedbazar, Udval;Yada, Toshihiko
通讯作者：
Yada, Toshihiko
影响因子：
5.3
作者：
Prakash, Nandkishore;Stark, Christiana J.;Dulcis, Davide
通讯作者：
Dulcis, Davide
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K