Fluorescent silica nanoparticles with efficient urinary excretion for nanomedicine.
Fluorescent silica nanoparticles with efficient urinary excretion for nanomedicine.
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DOI：
10.1021/nl803405h
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发表时间：
2009-01
期刊：
影响因子：
10.8
通讯作者：
Bradbury M
中科院分区：
文献类型：
--
作者：
Burns AA;Vider J;Ow H;Herz E;Penate-Medina O;Baumgart M;Larson SM;Wiesner U;Bradbury M
开发具有高生物稳定性、生物相容性和高效清除特性的分子靶向探针，对于优化生物分布以及跨生物屏障的转运至关重要。此外，将符合这些标准的探针与高灵敏度、定量成像策略相结合，对于确保体内早期肿瘤检测和及时的治疗反应是必不可少的。这些挑战往往只是单独进行研究，这阻碍了荧光探针的临床转化。通过同时优化这些设计标准，我们创造了新一代基于核 - 壳二氧化硅的近红外荧光纳米粒子（C点），其流体动力学直径调整为3.3纳米和6.0纳米，相较于母体染料具有更优的光物理特性。一种中性有机涂层可防止血清蛋白吸附，并促进高效的尿液排泄。使用更定量的离体荧光检测方案以及光学 - 正电子发射断层成像（PET）相结合的方法进行了详细的粒子生物分布研究。结果表明，新一代C点构成了一个有前景的可临床转化的材料平台，可适用于肿瘤靶向和治疗。
The development of molecularly targeted probes that exhibit high biostability, biocompatibility, and efficient clearance profiles is key to optimizing biodistribution and transport across biological barriers. Further, coupling probes designed to meet these criteria with high-sensitivity, quantitative imaging strategies is mandatory for ensuring early in vivo tumor detection and timely treatment response. These challenges have often only been examined individually, impeding the clinical translation of fluorescent probes. By simultaneously optimizing these design criteria, we created a new generation of near-infrared fluorescent core–shell silica-based nanoparticles (C dots) tuned to hydrodynamic diameters of 3.3 and 6.0 nm with improved photophysical characteristics over the parent dye. A neutral organic coating prevented adsorption of serum proteins and facilitated efficient urinary excretion. Detailed particle biodistribution studies were performed using more quantitative ex vivo fluorescence detection protocols and combined optical-PET imaging. The results suggest that this new generation of C dots constitutes a promising clinically translatable materials platform which may be adapted for tumor targeting and treatment.
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