Metal stressors consistently modulate bacterial conjugal plasmid uptake potential in a phylogenetically conserved manner.
Metal stressors consistently modulate bacterial conjugal plasmid uptake potential in a phylogenetically conserved manner.
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DOI：
10.1038/ismej.2016.98
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发表时间：
2017-01
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影响因子：
--
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中科院分区：
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--
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微生物群落中接合质粒转移的环境刺激剂和抑制剂知之甚少。具体来说，目前尚不清楚暴露于压力源是否会导致社区改变其质粒吸收能力。我们评估是否金属（铜，镉，镍，锌）和一个类金属（As），在浓度引起部分生长抑制，调节社区的宽容（即吸收能力）对广泛的主机范围IncP型质粒（pKJK5）。从农业土壤中提取细胞作为受体群落，并与外源E. coli供体菌株。供体含有gfp标记的pKJK 5衍生物，可以通过显微镜定量缀合事件，并通过FACS和16S rRNA扩增子测序以高分辨率分离和遗传学描述transconjugants。金属胁迫一贯降低质粒转移频率的社会，而transconjugal池丰富度保持不受影响的OTU属于12个细菌门。的transconjugal池的分类组成是不同的，从各自的受体社区和集群依赖于应力类型和剂量。然而，对于某些OTU，胁迫使质粒容许性降低超过1000倍，并且这种响应通常在不同金属和剂量之间相关。此外，对某些胁迫的反应在遗传上是保守的。这是第一次证明，社区宽容是敏感的金属（loid）压力的方式，这是部分一致的压力源和遗传保守。
The environmental stimulants and inhibitors of conjugal plasmid transfer in microbial communities are poorly understood. Specifically, it is not known whether exposure to stressors may cause a community to alter its plasmid uptake ability. We assessed whether metals (Cu, Cd, Ni, Zn) and one metalloid (As), at concentrations causing partial growth inhibition, modulate community permissiveness (i.e. uptake ability) against a broad-host-range IncP-type plasmid (pKJK5). Cells were extracted from an agricultural soil as recipient community and a previously described cultivation-minimal filter mating assay was conducted with an exogenous E. coli donor strain. The donor hosted a gfp-tagged pKJK5 derivative from which conjugation events could be microscopically quantified and transconjugants isolated and phylogenetically described at high resolution via FACS and 16S rRNA amplicon sequencing. Metal stress consistently decreased plasmid transfer frequencies to the community, while the transconjugal pool richness remained unaffected with OTUs belonging to 12 bacterial phyla. The taxonomic composition of the transconjugal pools was distinct from their respective recipient communities and clustered dependent on the stress type and dose. However, for certain OTUs, stress in- or decreased plasmid permissiveness by more than 1000-fold and this response was typically correlated across different metals and doses. The response to some stresses was, in addition, phylogenetically conserved. This is the first demonstration that community permissiveness is sensitive to metal(loid) stress in a manner that is both partially consistent across stressors and phylogenetically conserved.
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